FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001432580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30096815
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGAACTCCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT8359732.7776128470736854No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC6838272.272090917261511Illumina Single End PCR Primer 1 (96% over 31bp)
TGTTGAACTCCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA5321801.7682269701960158No Hit
TGTTGAACTCCGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC1902070.6319838162277304No Hit
TGTTGAACTCCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC883810.2936556575837011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)