FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001432581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33979839
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGAACTCCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT10523093.096862819155794No Hit
TGTTGAACTCCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA6712251.975362508339136No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG5731381.6867001635881795TruSeq Adapter, Index 6 (97% over 35bp)
TGTTGAACTCCGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2575700.7580083001570431No Hit
TGTTGAACTCCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC1387440.4083127056605536No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAACTCGTATG422410.12431194862341756TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)