FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001432582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33979839
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGAACTCCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT9904012.914672432673975No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC6527561.9210096904814646Illumina Single End PCR Primer 1 (96% over 31bp)
TGTTGAACTCCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA6318571.8595055732900914No Hit
TGTTGAACTCCGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2200650.6476340279305031No Hit
TGTTGAACTCCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC1021160.30051937562152664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)