FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001432583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23155651
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACGCAGTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT15584386.730270723116358No Hit
ATATACGCAGTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA9564134.130365412745252No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG6675812.8830154678009268TruSeq Adapter, Index 2 (97% over 36bp)
ATATACGCAGTGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC3467671.4975480499339018No Hit
ATATACGCAGTGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2285870.9871758733969518No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAACTCGTATG309450.13363908447229575TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)