FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001432584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23155651
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACGCAGTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT13544965.849526752670439No Hit
ATATACGCAGTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA9408474.063142081386526No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC7174113.0982113178333877Illumina Single End PCR Primer 1 (96% over 31bp)
ATATACGCAGTGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC2650221.1445240731949191No Hit
ATATACGCAGTGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC1981620.8557824610502205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)