FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001432585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27931716
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACGCAGTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT18705336.696806597919011No Hit
ATATACGCAGTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA11668944.17766670690766No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG6680812.391836577459115TruSeq Adapter, Index 2 (97% over 36bp)
ATATACGCAGTGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC4259711.5250441469475058No Hit
ATATACGCAGTGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2797981.0017214839217181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)