FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001432586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001432586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27931716
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACGCAGTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT16970256.075620273383848No Hit
ATATACGCAGTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA11587434.148484826352953No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC7362632.6359390164213328Illumina Single End PCR Primer 1 (96% over 31bp)
ATATACGCAGTGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC3285571.1762864837949807No Hit
ATATACGCAGTGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2448290.8765268843489601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)