FastQCFastQC Report
Mon 6 Mar 2023
EGAF00001435547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001435547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73200408
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG26458213.6144894165070776TruSeq Adapter, Index 18 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT2499280.3414297909377773TruSeq Adapter, Index 18 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCCCGTATG916390.12518919293455305TruSeq Adapter, Index 18 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)