FastQCFastQC Report
Mon 6 Mar 2023
EGAF00001435557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001435557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73808908
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG27703233.7533721539410934TruSeq Adapter, Index 18 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT2600840.3523748109103579TruSeq Adapter, Index 18 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCCCGTATG952910.12910501263614416TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCGCGTATG743620.10074935670366508TruSeq Adapter, Index 18 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)