FastQCFastQC Report
Mon 18 Sep 2023
EGAF00001552128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001552128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59534397
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT959810.16121940396910378TruSeq Adapter, Index 25 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA834250.1401290752974285TruSeq Adapter, Index 25 (97% over 44bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG716250.12030860075730673No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT687940.11555336656890974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)