FastQCFastQC Report
Mon 3 Apr 2023
EGAF00001552130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001552130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74854792
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1673100.22351274451473993No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1425530.19043937761526342No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT1131530.15116333500732992TruSeq Adapter, Index 27 (97% over 44bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1082040.14455186783499446No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT996280.1330950194878639No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA909060.1214431268475103TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)