FastQCFastQC Report
Fri 17 Mar 2023
EGAF00001554959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001554959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64018772
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC11609191.8134040434265124TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2495290.3897747366975424TruSeq Adapter, Index 7 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCCCGTATGCC753890.11776077179362328TruSeq Adapter, Index 7 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)