FastQCFastQC Report
Sat 18 Mar 2023
EGAF00001554997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001554997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65147370
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC12144941.8642256778746404TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2568330.3942338731402357TruSeq Adapter, Index 7 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCCCGTATGCC754100.11575294597464181TruSeq Adapter, Index 7 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)