FastQCFastQC Report
Wed 27 Sep 2023
EGAF00001561169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001561169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33692320
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3173530.9419149527251314Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1394620.4139281592956495Illumina Single End PCR Primer 1 (100% over 50bp)
GTACTCCATATTATAATATTGTTTAAAACTATTTTTAAACACATTAAAAA930710.276238026945013No Hit
GGAACCTCGAGCATATGCTACCGGACAGCGGTGCGGACTGTTGTAACTCA706230.20961156726518093No Hit
CCAATGAAGACAAGTCCTCCACCTAAACCATGTCCTCCATCCAAACCATG605230.1796344092659692No Hit
GGATATCTGGGTAGTAACGGGATACTAGGTAGTGGTTTGGGTGGAGAATA604350.17937322214676815No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCTGAAGAGCGTCGTGTAGG480150.1425102219140742Illumina Single End PCR Primer 1 (100% over 33bp)
CAATGAAGACAAGTCCTCCACCTAAACCATGTCCTCCATCCAAACCATGT445260.13215474624484155No Hit
ACACTCTTTCCCTACAGACGCTCTTCCGATCTGTAGGGAAAGAGTGTAGA371090.11014082734581648No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)