FastQCFastQC Report
Wed 22 Feb 2023
EGAF00001561170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001561170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95053741
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGAAGACAAGTCCTCCACCTAAACCATGTCCTCCATCCAAACCATGT16684271.755246013936474No Hit
CAATGGTCTCCACCTAAACCGTGTTCTCCACCCAAACCGTGTCGTCCACC8798830.9256689855057888No Hit
GATATCACACAACCACTCAACCCATTTCCCCTACCTAAACCATGCCCGCC7065360.7433016234468878No Hit
GATATCTGGGTAGTAACGGGATACTAGGTAGTGGTTTGGGTGGAGAATAG6893400.7252108046962613No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAACGTATCTCGTAT3222880.339058722580945TruSeq Adapter, Index 22 (97% over 37bp)
TGGTCTCGAGGTGGAGGACATGGTTTGGGTGGCGGGCATGGCTTGGGTGG3026240.3183714778779722No Hit
TTGAAGACAGGGACATGGTTTAGGTGGAGGACATGGTTTAGGTGGAGGAC2687070.2826895576892655No Hit
TGGTCTCGTCGTTGTTGCTGCTGTTGCTGTTGCTGTTGTCGTTATTGTTG950600.10000658469612468No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)