FastQCFastQC Report
Thu 9 Feb 2023
EGAF00001561171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001561171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95053741
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATCTGGGTAGTAACGGGATACTAGGTAGTGGTTTGGGTGGAGAATAG12090041.2719162731322695No Hit
CAATGAAGACAAGTCCTCCACCTAAACCATGTCCTCCATCCAAACCATGT6952510.7314293921372332No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3390030.35664351180034043Illumina Single End PCR Primer 1 (100% over 50bp)
GATATCACACAACCACTCAACCCATTTCCCCTACCTAAACCATGCCCGCC2637070.27742937545193513No Hit
CAATGGTCTCCACCTAAACCGTGTTCTCCACCCAAACCGTGTCGTCCACC2564210.26976423789569737No Hit
GGATATCTGGGTAGTAACGGGATACTAGGTAGTGGTTTGGGTGGAGAATA2338930.2460639608071817No Hit
TGTCAGGTACAATTCGTACTACGACAACAACAATAACGACAACAGCAACA1129100.11878543528339405No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)