FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001561310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001561310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21113516
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC1499380.7101517340835132Illumina Single End PCR Primer 1 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCTTGTATCTCGTA401680.19024780145571207TruSeq Adapter, Index 13 (97% over 37bp)
GGATATCTGGGTAGTAACGGGATACTAGGTAGTGGTTTGGGTGGAGAATA369590.17504900652264643No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)