FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001561324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001561324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15191362
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC3202652.1082046494580275Illumina Single End PCR Primer 1 (100% over 33bp)
GGATATCTGGGTAGTAACGGGATACTAGGTAGTGGTTTGGGTGGAGAATA646630.4256563697185282No Hit
GGAACCACGATACTATGACTGAGAGTCAACGCCATGAGCGGCCTCATTTC359640.23673979989417673No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCGTAGATCTCGTA270370.17797614196804737TruSeq Adapter, Index 9 (97% over 37bp)
ATTGAAGACAGGGACATGGTTTAGGTGGAGGACATGGTTTAGGTGGAGGA214020.14088269373081888No Hit
GGATATCACACAACCACTCAACCCATTTCCCCTACCTAAACCATGCCCGC191010.1257359280886072No Hit
CAATGAAGACAAGTCCTCCACCTAAACCATGTCCTCCATCCAAACCATGT186770.12294486827448387No Hit
CCAATGAAGACAAGTCCTCCACCTAAACCATGTCCTCCATCCAAACCATG180090.1185476325295915No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAG166240.1094306093160047Illumina Single End PCR Primer 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)