FastQCFastQC Report
Sun 5 Mar 2023
EGAF00001561450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001561450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86427012
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGAAGACAAGTCCTCCACCTAAACCATGTCCTCCATCCAAACCATGT5197420.6013652305832348No Hit
GATATCTGGGTAGTAACGGGATACTAGGTAGTGGTTTGGGTGGAGAATAG3372280.39018819718076103No Hit
GATATCACACAACCACTCAACCCATTTCCCCTACCTAAACCATGCCCGCC2798700.3238223716446427No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAGTATCTCGTAT2607740.30172742753156845TruSeq Adapter, Index 7 (97% over 35bp)
CAATGGTCTCCACCTAAACCGTGTTCTCCACCCAAACCGTGTCGTCCACC2033120.23524126924577698No Hit
TGGTCTCGAGGTGGAGGACATGGTTTGGGTGGCGGGCATGGCTTGGGTGG1107680.12816363476733408No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCACGTAGTATCTCGTATGCCGT880730.10190448328816458TruSeq Adapter, Index 7 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)