FastQCFastQC Report
Tue 19 Sep 2023
EGAF00001561572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001561572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39456947
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC2330490.590641237397308Illumina Single End PCR Primer 1 (100% over 33bp)
GGATATCTGGGTAGTAACGGGATACTAGGTAGTGGTTTGGGTGGAGAATA596620.15120784687168018No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCGGTATCTCGTA560830.14213720083310044TruSeq Adapter, Index 14 (97% over 38bp)
GTACTCCATATTATAATATTGTATAAAATGATAATAATAGTTATTTTTTT511300.12958427827677593No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCGGTATCTCGTAT473790.12007771407149163TruSeq Adapter, Index 14 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)