FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820148
Sequences flagged as poor quality0
Sequence length35-300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG20086524.491311324297566No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG16810220.496544526109922No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGGCTAA557006.791457151636046No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCA353884.31483098172525No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTCCGGGTGTAACCC250613.055668001385116No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTATTACAACTCCAA247773.0210401049566666No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC245972.999092846656945No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC173422.1144964079653916No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGATAGGGTCCTTCGGG167132.0378029331291425No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG162551.981959353677629No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTAATCCAACTCAACTC120641.4709540229324465No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAATGAGAACGTGCCCTCACGG118731.4476655432921863No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGAA92611.1291864395206719No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGGGCT86581.0556631242166048No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAAC86231.0513956017694368No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA84311.0279851929164003No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAACATCAAACG80850.9857976852958247No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC74500.9083726351829182No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAGAGTTCATGCCCGAAAGG63690.7765671561718128No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTTACATGCAGTC60430.7368182328067617No Hit
TCCGTAGGTGAACCTGCGGAGAGGGCATCGATGAAGAACGCAGC59040.7198700722308656No Hit
TCCGTAGGTGAACCTGCGGGGGGATCATTACAAGAACGCCCGGGCTTCGG56670.690972848802899No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAATGCCCGGGCTTCGG53340.6503704209484141No Hit
TCCGTAGGTGAACCTGCGGAGGGATCACTAATGAGAACGTGCCCTCACGG52270.6373239951813575No Hit
TCCGTAGGTGAACCTGCGGAGGGAACATTACAAGAACGCCCGGGCTTCGG50770.6190346132649229No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGGGATAGGGTCCTTCGGG47930.5844067168364734No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTACCTTCGGGCTAA36070.43979867048386395No Hit
TCCGTAGGTGAACCTGCGGATCGAAAACGTGGGTCGGAGCGGGCATCGATGAAGAACGCAGC33350.40663392460872916No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACC31850.38834454269229457No Hit
TCCGTAGGTGAACCTGCGGAGGCATCGATGAAGAACGCAGC30930.3771270551168813No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGCACGCCCGGGCTTCGG28240.3443280968800753No Hit
TCCGTAGGTGAACCTGCGGACGAGGCATCGATGAAGAACGCAGC27420.33432990143242436No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCAAAGCAGAA26060.31774752849485705No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21800.2658056838521828No Hit
TCCGTAGGTGAACCTGCGGCATGCGTCCCGAGGAAAGGAGTTCGGCGTGG21100.25727063895784663No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAAGAACGCCCGGGCTTCGG9770.11912484088237732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)