FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820148
Sequences flagged as poor quality0
Sequence length35-300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT26150731.885342645473745No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT23011828.05810658564064No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA482615.884425737793666No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA238432.907158220223667No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG194382.3700600379443713No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA173112.1107166023693282No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG165642.0196354804254844No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA161571.9700102908255583No Hit
GCTGCGTTCTTCATCGATGCCAGAACCTAGAGATCCGTTGTTGTAAGTTT117411.4315708872057238No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGGTCCGTTGTTGAAAGTTA102141.2453849792964198No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCATCGTTGAGAATTT98531.2013685334842004No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG95041.1588152382252959No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT92321.1256504923501613No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGCTT63750.7772987314484703No Hit
GCTGCGTTCTTCATCGATGCCCTCTCCGCAGGTTCACCTACGGA58950.7187727093158796No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGATATCCGTTGCCGAGAGTCA58860.7176753464008935No Hit
GCTGCGTTCTTCATCGATGCTAGAACCAAGAGATCCGTTGTTAAAAGTTT55480.6764632724825276No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTCGTTAAAAGTTT45130.5502665372591288No Hit
GCTGCGTTCTTCATCGATGCCCGCTCCGACCCACGTTTTCGATCCGCAGGTTCACCTACGGA32300.39383135726722496No Hit
GCTGCGTTCTTCATCGATGCCTCCGCAGGTTCACCTACGGA28560.3482298316889147No Hit
GCTGCGTTCTTCATCGATGCCTCGTCCGCAGGTTCACCTACGGA27080.3301843081980325No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTCAAAGTTT26230.31982032511205294No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22400.2731214366187566No Hit
GCTGCGTTCTTCATCGATGCGGAGCTTGTCGGGGTTGACGGTGCCGCCCG19330.2356891682964538No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT14450.17618771246165327No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTAAAAGTTT13250.16155620692850559No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTA12660.15436238337470798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)