FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989072
Sequences flagged as poor quality0
Sequence length35-300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGGCTAA38593539.01990957180064No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC486274.916426711098889No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCA453864.588745814258214No Hit
TCCGTAGGTGAACCTGCGGAGGGATCACTAATGAGAACGTGCCCTCACGG443754.486528786579743No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG399414.038229774980992No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAATGAGAACGTGCCCTCACGG392003.9633110632997397No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACC284952.880983386447094No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTATCGAGTTATTCAACTCCCAA153161.548522251160684No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC142611.4418566090234077No Hit
TCCGTAGGTGAACCTGCGGAGGGGTCATTACAAGTGACCCCGGTTTACCA136751.3826091528220392No Hit
TCCGTAGGTGAACCTGCGGACGGCATCGATGAAGAACGCAGC108561.0975945128362747No Hit
TCCGTAGGTGAACCTGCGGACCCCAAGTACCATGAAGATGGGGATGGGAT92320.9334001973567142No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGAGTTTGACATGGATTGT85820.8676820292152644No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTGAGGGCCCTCCGGC84910.8584814856754615No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAACCTGCGCAGCAGAAC83860.8478654738987658No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATAATAGCGAAAGCTTTCAT83830.8475621592765744No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGAA74290.7511081094197389No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGTCTAA73420.742311985376191No Hit
TCCGTAGGTGAACCTGCGGAGGAGGAGAGCATAGGCATCGATGAAGAACGCAGC71840.7263374152741155No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTCGCCTTCGGGCTAA69000.6976236310400051No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTAGGGGTCCTCCGG66770.6750772441237847No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG66770.6750772441237847No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACC60670.6134032709448858No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA59840.6050115663975929No Hit
TCCGTAGGTGAACCTGCGGAGTAGTGGCATCGATGAAGAACGCAGC58070.5871160036883057No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAATGCCTGGGCTGTCAT55330.5594132681948331No Hit
TCCGTAGGTGAACCTGCGGACCTACCGCTGGCAGTGCATCGATGAAGAACGCAGC53010.5359569374120388No Hit
TCCGTAGGTGAACCTGCGGAGTGATCACTAATGAGAACGTGCCCTCACGG48530.49066195383147027No Hit
TCCGTAGGTGAACCTGCGGAGGGGTCATTAATGAGAACGTGCCCTCACGG48400.4893475904686413No Hit
TCCGTAGGTGAACCTGCGGAGGGATCACTAATGAGGACGTGCCCTCACGG44790.45284873093162076No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAATGAGAAAGTGCCCTCACGG44650.451433262694728No Hit
TCCGTAGGTGAACCTGCGGACCGTTCTGCATCGATGAAGAACGCAGC43590.4407161460439685No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTAGTTTTACCTGCG41430.4188774932461944No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTGAGGGAGCAATCCC40100.4054305449957132No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGACTTCGGGCTAA38510.3893548700195739No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAAC30460.30796544639824No Hit
TCCGTAGGTGAACCTGCGGAGAGTTGGACAAAATGCATCGATGAAGAACGCAGC29690.30018037109532975No Hit
TCCGTAGGTGAACCTGCGGACAGAGCCCAAACGTGGAAGTGCATCGATGAAGAACGCAGC29550.298764902858437No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCCTCGG29100.2942151835255674No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG28970.2929008201627384No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAACATATATCGGGGGCCGGA26070.2635804066842454No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTGATGAGTATAAACAGGACTT25090.2536721290259961No Hit
TCCGTAGGTGAACCTGCGGAGCCGATTTCCATTCATGGAGCTGGGGGGAGTGCATCGATGAAGAACGCAGC24580.2485157804487439No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACACAATTAAACACCTTAAA24050.2431572221233641No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGGCCCCGGTTTACCA23220.2347655175760713No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTTTAACTCTCCAA21210.21444343788925377No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAATTTAGATTGAACCAT21120.21353349402267985No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTATAAGTGACCCCGGTTTACCA20390.2061528382160247No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTTGACCCCGGCCCTCG19490.1970533995502855No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGATAGGGTCCTTCGGG18400.1860329682773347No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA14450.146096543022146No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGGCTAC11000.11121536147014575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)