FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989072
Sequences flagged as poor quality0
Sequence length35-300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA37087637.49737127327435No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT19883420.10308652959542No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT474054.792876554992962No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA469384.745660578805183No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT299893.0320340682983646No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG230182.327231991199832No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG151751.5342664639176926No Hit
GCTGCGTTCTTCATCGATGCCGTCCGCAGGTTCACCTACGGA108181.0937525276218516No Hit
GCTGCGTTCTTCATCGATGCTGCTCAGCATCCCATCCCCATCTTCATGGT93280.9431062652668359No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG90090.9108538104404937No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA88850.8983168060565865No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT84760.8569649125645049No Hit
GCTGCGTTCTTCATCGATGCGATAGCCAAGAGATCCGTTGTTGAAAGTTA83710.8463489007878091No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTGAAAGTTA82840.8375527767442613No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT82740.8365417280036236No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGTGATCCGTTGTTGAAAGTTA76130.7697114062474724No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGGTCCGTTGTTGAAAGTTA74070.748883802190336No Hit
GCTGCGTTCTTCATCGATGCCTATGCTCTCCTCCTCCGCAGGTTCACCTACGGA70390.711677208534869No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA67850.6859965705226717No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAGGTTA67130.6787170195900804No Hit
GCTGCGTTCTTCATCGATGCCACTACTCCGCAGGTTCACCTACGGA57360.5799375576297782No Hit
GCTGCGTTCTTCATCGATGCACTGCCAGCGGTAGGTCCGCAGGTTCACCTACGGA49420.49966028762314574No Hit
GCTGCGTTCTTCATCGATGCAGAACGGTCCGCAGGTTCACCTACGGA41150.41604655677240887No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATACGTTGTTAAAAGTTT38100.38520957018295937No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTA32970.33334276978824595No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG29420.297450539495608No Hit
GCTGCGTTCTTCATCGATGCATTTTGTCCAACTCTCCGCAGGTTCACCTACGGA29240.2956306517624602No Hit
GCTGCGTTCTTCATCGATGCACTTCCACGTTTGGGCTCTGTCCGCAGGTTCACCTACGGA28340.28653121309672097No Hit
GCTGCGTTCTTCATCGTCACGGGAACCTAGATATCCACCGTTGAAAATTT27220.27520746720157885No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCTTTGTTAAAAGTTT26330.26620913340990343No Hit
GCTGCGTTCTTCATCGATGCACTCCCCCCAGCTCCATGAATGGAAATCGGCTCCGCAGGTTCACCTACGGA24010.24275280262710902No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTA18370.1857296536551434No Hit
GCTGCGTTCTTCATCGATGCCAGAAACAAGAGATCCGTTGTTGAAAGTTT17580.17774236860410567No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTA12760.1290098193053691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)