FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892651
Sequences flagged as poor quality0
Sequence length35-300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG19233321.54627060295681No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTCCGGGTGTAACCC14122415.82074069261111No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC10495511.757674611914398No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGGCTAA789258.841641358156771No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACGAAATAGGCCGGCGGCCCT695397.790166593663145No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGATTCCATCGTATCCACACCG609996.833465710563255No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGAAGCGGTTTCGGCCG595196.667667431056483No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAAGAGACAGGACCCCTCGG377204.225615610132067No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC162111.8160512899218173No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA161671.8111221518824265No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC56550.6335062639262152No Hit
TCCGTAGGTGAACCTGCCGAAGGATCATTAGTGAAGATTCAAGGGCCAGC43780.4904492349193582No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG25430.28488177350386656No Hit
TCCGTAGGTGAACCTGCGCATCGATGAAGAACGCAGC23350.26158039368129316No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGACGATTCAAGGGCCAGC22050.24701703129218475No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC18260.20455922863470716No Hit
TCCGTAGGTGAACCTCGGAAGGATCATTACGAAATAGGCCGGCGGCCCTG14390.16120521906097682No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13690.15336340854376457No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACGAACTAGGCCGGCGGCCCT13150.147314011859058No Hit
TCCGTAGGTGAACCTGCGGGGCAGAGGCGGCGGCCGCAGCGGCGCAGCTG11850.13275064946994963No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC10660.11941957159068886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)