FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001565385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892651
Sequences flagged as poor quality0
Sequence length35-300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA19156721.460458790725603No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT13765915.421368485555945No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG12741114.273327425836078No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA798198.941792481048024No Hit
GCTGCGTTCTTCATCGATGCTGGAACCAAGAGATCCGTTGTTGAAAGTTT605626.784510407762944No Hit
GCTGCGTTCTTCATCGATGCGGGAGCCAAGATATCCGTTGTTGAGAGTTG586066.565387816739129No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT585366.557546006221917No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT383704.298432422077609No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA160851.801936030990835No Hit
GCTGCGTTCTTCATCGACGCTGGAACCAAGAGATCCGTTGTTGAAAGTTT92571.0370234279690496No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA58200.6519905315739298No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC49830.5582248829609779No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG25720.28813052357528307No Hit
GCTGCGTTCTTCATCGATGCGCAGGTTCACCTACGGA21940.2457847467823371No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14200.15907672763487635No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTCAAAGTTT13680.15325138267923297No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA10200.11426638182223511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)