FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864091
Sequences flagged as poor quality0
Sequence length35-300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGGCTAA36731142.508370067504465No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGATAGGGTCCTTCGGG9584111.091540127139385No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAATGAGAACGTGCCCTCACGG444935.149110452487064No Hit
TCCGTAGGTGAACCTGCGGAGGGATCACTAATGAGAACGTGCCCTCACGG373194.318873822317325No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCA187802.173382201643114No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG149101.7255127064163382No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTAATCCAACTCAACTC138951.6080482264020801No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC134951.5617568057068063No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCAGGCTAA129491.4985690164577574No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA123931.4342239416913265No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACTTCCGGGCTTCGG101131.1703628437282647No Hit
TCCGTAGGTGAACCTGCGGAACGCCAACTCCGCTACTGTCTACTAGGCTC100381.161683202347901No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACC98461.1394633204141693No Hit
TCCGTAGGTGAACCTGCGGAGGGGTCATTACAAGTGACCCCGGTTTACCA98431.1391161347589547No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG93101.0774328166825022No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG91451.0583376056457017No Hit
TCCGTAGGTGAACCTGCGGAGGACAATTCGGCAGTGGCAGTGGTAGTTGT88471.0238504972277225No Hit
TCCGTAGGTGAACCTGCGGAGTAGTGGCATCGATGAAGAACGCAGC77770.9000209468678646No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC75060.8686585093468165No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTTTTTAACTCTCCAA74600.86333499596686No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTATTGGGTCTTAGTG65780.7612624133337808No Hit
TCCGTAGGTGAACCTGCGGAATGATCATTAAAGAGATAGGGTCCTTCGGG59600.6897421683595825No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAACGTCTTGCCTTTAGTTAG55380.6409047195260684No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTAGGGTCTCTTCAG54740.6334980922148247No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTGGCTGGAATTTATTC40790.4720567625400566No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39470.45678059371061613No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGAGCGT29140.33723299976507104No Hit
TCCGTAGGTGAACCTGCCCATGCATCGATGAAGAACGCAGC25730.29776956362234996No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACAAATAATGAAAACACC18950.21930560554386055No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC18630.2156022918882386No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACTGAGTGTAAAAACTCCCAA14560.16850077133079733No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTAGTTTTACCTGCG14260.16502891477865178No Hit
TCCGTAGGTGAACCTGCGTAAGGATCATTAGTGATTTGCCTTCGGGCTAA11320.1310047205676254No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA10380.12012623670423601No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGGCTAC10110.11700156580730502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)