FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864091
Sequences flagged as poor quality0
Sequence length35-300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA36860542.65812281345367No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT13810515.982691637802038No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT12856714.878872711323229No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG138371.6013359704012655No Hit
GCTGCGTTCTTCATCGATGCGGGAGCCAAGAGATCCGTTGTTGAAAGTTA131541.5222933695640852No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA129551.4992633877681865No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCATCGTTGAGAATTT121651.4078378318950204No Hit
GCTGCGTTCTTCATCGATGCTGGCAGAGGGTAAGAGCCTAGTAGACAGTA93661.0839136155798406No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA92471.0701419179229965No Hit
GCTGCGTTCTTCATCGATGCCCGAAACCAGAACAACTACCACTGCCACTG86230.9979273016383692No Hit
GCTGCGTTCTTCATCGATGCCACTACTCCGCAGGTTCACCTACGGA76540.8857863350040679No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA74680.8642608243807655No Hit
GCTGCGTTCTTCATCGATGCCAGAACCTAGAGATCCGTTGTTAAAAGTTT67850.7852182235435851No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTG53340.6172960949714786No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39790.460483907366238No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATACGTTGTTAAAAGTTT29570.34220932748981303No Hit
GCTGCGTTCTTCATCGATGCATGGGCAGGTTCACCTACGGA23930.2769384243094767No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT21310.24661754375407222No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT21230.2456917153401667No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTA20900.2418726731328066No Hit
GCTGCGTTCTTCATCGTTGTGGGAACCAAGATATCCATTGCTAAGAGTTG19720.22821670402770078No Hit
GCTGCGTTCTTCATCGATGTGGGAACCAAGATATCCATTGCTAAGAGTTG18780.2173382201643114No Hit
GCTGCGTTCTTCATCGATATGGGAACCAAGATATCCACCGTTGAAAATTT17740.20530245078354015No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA17200.19905310898967818No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT16840.1948868811271035No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTA15290.17694895560768484No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTCAAAGTTT13300.15391897381178604No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTA12240.1416517473275384No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTA10060.1164229230486141No Hit
GCTGCGTTCTTCATCGATGCGAGATCCAAGAGATCCGTTGTTGAAAGTTA9980.11549709463470861No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)