FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001565388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690919
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG9088713.154508705072521No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGAGGCTCTACGCTTGATG598468.661796824229757No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC411725.959019798268683No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAAATTATCGTGTTCTTTGG307514.450738798614599No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAAATTCAAGGACGCTCT284114.112059445463216No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA269713.9036413819854427No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC233403.3781094455355842No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGAAGTTGCTTGAGGGGTT229993.3287548902259165No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN216583.1346655686122396No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC214253.1009423680634054No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGGGCT201722.9195897058844813No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATCGT183462.6553040226133597No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACATATCCAATCCAACCTAA175832.5448713959234004No Hit
TCCGTAGGTGAACCTGCGAATACCTCGATGTTGGGCCTCCCAGTTTCCAG168452.4380571383910414No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAGGCGGGCTG150802.1826002758644645No Hit
TCCGTAGGTGAACCTGCGGAAGGATCACTAACACTAATCCACAAATCTTA143112.071299240576681No Hit
TCCGTAGGTGAACCTGCGCATCGATGAAGAACGCAGC141572.0490100865658634No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGAGTTTTGAACGGGTTGT131621.904998994093374No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATCAT124801.8062898834740395No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG117911.7065676294905772No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC96511.3968352295999966No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG91711.327362541774072No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATTTATGCAATGTTCTT89101.2895867677687254No Hit
TCCGTAGGTGAACCTGCATCCCAGGGATTAAGCACACTTGATCTTGGTGG81961.1862461446276626No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGAGATAAAGCTGACTTGATCGT78871.1415231018397236No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAGTTCCTTTTCGAACAG60180.8710138236175298No Hit
TCCGTAGGTGAACCTGCGGATGTAGAAATGAAGAACGCAGC55370.8013964010253011No Hit
TCCGTAGGTGAACCTGCTCATCGATGAAGAACGCAGC53060.7679626700090748No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAAATTATCGTGTTCTTTTG48360.6999373298461903No Hit
TCCGTAGGTGAACCTGCGGGGCATGGCCTTCTTCGGCCTGACCGAGTTCC42540.6157016958572568No Hit
TCCGTAGGTGAACCTGCGTGCCTGTGGCTCACTCCAGGGGTGTGTTGCGT41450.5999256063301197No Hit
TCCGTAGGTGAACCTGCGGCCCCGGGCTGTCTTCCGCAGCTGGAAGCCCT40010.5790837999823424No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA38130.5518736639171885No Hit
TCCGTAGGTGAACCTGTAGGGATTGGGCAGCTCCGTGAGTGTCTCTGTCG35590.5151110332759701No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC29590.42827017349356433No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTCATAATCATGTTTGTTCTTCT28120.40699416284687495No Hit
TCCGTAGGTGAACCTGCGTGACACATTCGATGAAGAACGCAGC22370.3237716722220695No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG21870.316534933906869No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGAATGAAGCCCACTTGATCA17960.25994364028200123No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCTAGCG17220.2492332675755045No Hit
TCCGTAGGTGAACCTGCGGCGTGTTTGCTCCCCTCTGGGCTGTGAGACCC16790.24300967262443207No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCAACTTGTTCGT14240.20610230721690967No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA14110.20422075525495753No Hit
TCCGTAGGTGAACCTGCGGGAGCTGTACCTTAGCCACAATGGCATCGATGAAGAACGCAGC13860.20060238609735728No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCAACTTGATCGT12700.18381315320609218No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGACTGT12020.17397118909741952No Hit
TCCGTAGGTGAACCTGCCGCCCGCCGCCAGAGGCCGAGGCCAGACGATCT11940.17281331096698746No Hit
TCCGTAGGTGAACCTGCGTGCTCCTGTACTGGGTGCATATACATTTAGGA11670.16890547227677918No Hit
TCCGTAGGTGAACCTGCATGAGGCAGCGGTCCCTCAGAGAGTCTGTTTCT10440.151103096021386No Hit
TCCGTAGGTGAACCTGCGGGGCAGAGGCGGCGGCCGCAGCGGCGCAGCTG9050.13098496350512867No Hit
TCCGTAGGTGAACCTGCCTTGCATCCTGGGGTGAAGCCAACTTGATCGTG9040.13084022873882467No Hit
TCCGTAGGTGAACCTGCTGAGGCCAGGAGAAGGAGGTCATAGGGCAGCAG7570.10956421809213525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)