FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690919
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA8839012.793105993611405No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG8105511.731476482771496No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG502897.278566662662338No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC405755.872613142785188No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG352415.1005978993196015No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCCGAAAGTTT282074.082533553137199No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG280254.056191825669869No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA237203.4331086567311075No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN217043.141323367862224No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGAGATCCATTGTTAAAAGTTG183592.657185574575312No Hit
GCTGCGTTCTTCATCGATTTGTGCTGCTATAACAATATACGACAGAGTGA169462.4526753497877465No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGATATCCATTGTTAAAAGTTG167742.427780969983457No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCACTGTTGAAAATTT162812.35642673019558No Hit
GCTGCGTTCTTCATCGATGCGCAGGTTCACCTACGGA141942.054365272919112No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCATCGTTGAGAATTT139802.023392032930054No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG132891.9233803094139836No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAAATTT116521.6864494969743196No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG96461.3961115557684765No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT91231.3204152729914793No Hit
GCTGCGTTCTTCATCGATGCAAACATTCTCAATAAAATACTGGCAAACCG83901.2143246892906405No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG79731.1539702917418686No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATATGGGCAAATTG78311.1334179549266992No Hit
GCTGCGTTCTTCATCGACACGAGAGCCAAGATATCCATTGCCAGGAGTCA58180.8420668703567278No Hit
GCTGCGTTCTTCATTTCTACATCCGCAGGTTCACCTACGGA56490.8176066948513502No Hit
GCTGCGTTCTTCATCGATGAGCAGGTTCACCTACGGA53690.7770809602862275No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCA45750.6621615558408438No Hit
GCTGCGTTCTTCATCGATGCGCCGGATGGTGTCTTCATCCACCTCCACGC41970.6074518141779282No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC39940.5780706566182143No Hit
GCTGCGTTCTTCATCGATGCCCTGCTGGCTGACATCAGGAAGGGCTTCCA39490.5715575921345338No Hit
GCTGCGTTCTTCATCGATGCCTCCAATCACAGCCAGGACTGCCATGAGGC37440.5418869650422119No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG37380.5410185564443879No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA31160.4509935318032939No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA29570.42798070396095633No Hit
GCTGCGTTCTTCATCGAATGTGTCACGCAGGTTCACCTACGGA22560.32652163278184565No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC21640.31320603428187677No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT17820.25791735355374507No Hit
GCTGCGTTCTTCATCACAGACAATTGCTGAGAACTGACCCTGGGCTCACA15890.22998354365707124No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG15200.21999684478209458No Hit
GCTGCGTTCTTCATCGATGCAAAAGTCCTCAACAAAATACTGGCAAACCA14110.20422075525495753No Hit
GCTGCGTTCTTCATCGATGCCATTGTGGCTAAGGTACAGCTCCCGCAGGTTCACCTACGGA13940.20176026422778934No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAAACA12830.1856947051680443No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAAAAAAATACTACCAAACTG11960.17310278049959546No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCACTGTTGAAAATTT11810.17093175900503532No Hit
GCTGCGTTCTTCATCGATGCTAGGAAGAAACTGCATCAACTAACGAGCAA11370.1645634292876589No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG10580.15312938274964213No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATATGCCAAACCAA8880.1285244724779605No Hit
GCTGCGTTCTTCAAATGCGGGGGCAGGCGTGAAGCTGGCCCGACGCACCC8410.12172193846167206No Hit
GCTGCGTTCTTCATCGATGCCACTGTGCTTTCTCCCAGCCAGGCCCTACT7430.10753793136387911No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)