FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001565390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722605
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGAGGCTCTACGCTTGATG12830117.75534351409138No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN601928.329862096165956No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG492016.808837469987061No Hit
TCCGTAGGTGAACCTGCGTGCCTGTGGCTCACTCCAGGGGTGTGTTGCGT427505.916095238754229No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC397325.498439673127089No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC348774.826564997474415No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC347964.815355553864144No Hit
TCCGTAGGTGAACCTGCGGGGATCCTAAGAAAGCCCCAAAGGAAACCTGC216412.9948588786404744No Hit
TCCGTAGGTGAACCTGCATGAGGCAGCGGTCCCTCAGAGAGTCTGTTTCT214132.963306370700452No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATCAT194022.685007715141744No Hit
TCCGTAGGTGAACCTGCATCCCAGGGATTAAGCACACTTGATCTTGGTGG146402.026003141411975No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA141031.9516886819216583No Hit
TCCGTAGGTGAACCTGTAGGGATTGGGCAGCTCCGTGAGTGTCTCTGTCG135271.8719770829152858No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATCGT120261.6642564056434705No Hit
TCCGTAGGTGAACCTGCGGCCCCGGGCTGTCTTCCGCAGCTGGAAGCCCT104941.4522456943973538No Hit
TCCGTAGGTGAACCTGCGGTGTTTGCGTGGCATCGATGAAGAACGCAGC89941.2446634053182584No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC82921.1475148940292415No Hit
TCCGTAGGTGAACCTGCGTTGCATCCCAGGAATGAAGCCCACTTGATCAT82471.1412874253568686No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG68660.950173331211381No Hit
TCCGTAGGTGAACCTGGTCTCCGAGCACATCTGGTGTGAGGACTTCCTGG64660.8948180541236223No Hit
TCCGTAGGTGAACCTGCGAATACCTCGATGTTGGGCCTCCCAGTTTCCAG61900.8566229129330685No Hit
TCCGTAGGTGAACCTGCAAACCAAAATGTTTTACTCCCTGTCTCTCTTTC57220.7918572387403906No Hit
TCCGTAGGTGAACCTGCCTTGTATCCCAGGGATTAAGCCAACTTGATTTT54100.7486801226119387No Hit
TCCGTAGGTGAACCTGCCTTGTATCCCAGGGATGAAGCCGACTTGATTGT53750.7438365358667599No Hit
TCCGTAGGTGAACCTGCCTTGCATCCTGGGGTGAAGCCAACTTGATCGTG50650.7009361961237468No Hit
TCCGTAGGTGAACCTGCCCCTGCCAGGGTTTCAGCAGGGCCAAGCTGGAA45240.6260681838625528No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAACAGAGAAGAGCAAAGCCAC44410.6145819638668429No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGAGATAAAGCTGACTTGATCGT37990.5257367441409899No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGGTCAT35870.4963984472844777No Hit
TCCGTAGGTGAACCTGCGGCTTCCTGGGAGAGAGGAGAAAAGTCAAAGGC33030.4570962005521689No Hit
TCCGTAGGTGAACCTGCGGGGGTCCTGGGGCAGGTGATTGATTGCCAACA33000.45668103597401066No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGAAGAAGCCGACTTGATCAT29640.41018260322029326No Hit
TCCGTAGGTGAACCTGCCACCTGGCCCTTGAGCCTAGAATGGCATCAGGG26490.3665903225136831No Hit
TCCGTAGGTGAACCTGCAGCAGTAACCACACAGGTGGACCTCTTAGGTTGGAGCTGGATGAAGAACGCAGC24820.3434794943295438No Hit
TCCGTAGGTGAACCTGCTGAGGCCAGGAGAAGGAGGTCATAGGGCAGCAG18590.25726365026535936No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG17620.24383999557157782No Hit
TCCGTAGGTGAACCTGCGGCGTGTTTGCTCCCCTCTGAGCTGTGAGACCC15870.2196220618456833No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCACGAATGAAGCTGACTTGATTGT12150.1681416541540676No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGACGAAGCCCACTTGATCAT11650.16122224451809772No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC10660.14752181343887738No Hit
TCCGTAGGTGAACCTGTTCTCCGAGCACATCTGGTGTGAGGACTTCCTGG10310.1426782266936985No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATTGT10290.1424014503082597No Hit
TCCGTAGGTGAACCTGCGGGGACAAGGGCATGGGGTGGTAGGAGAAAAGG9970.137973028141239No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACCGCATCGATGAAGAACGCAGC9730.13465171151597347No Hit
TCCGTAGGTGAACCTGCGGCAGGAAGCACAGGACAGTGGCACAGGAAGCA9660.13368299416693766No Hit
TCCGTAGGTGAACCTGGCAGGATTCCAACCCCCAGCTCAATAATACTGTA8970.12413420886929927No Hit
TCCGTAGGTGAACCTGCACCGCATCGATGAAGAACGCAGC8460.11707641104061002No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGCGGCGGAACAGAGGGGAC8200.11347831802990568No Hit
TCCGTAGGTGAACCTGCGTGAGGCAGCGGTCCCTCAGAGAGTCTGTTTCT7880.10904989586288498No Hit
TCCGTAGGTGAACCTGCGGGGATCCTAAGAAAGCCCCAAAGGCAACCTGC7750.10725084935753282No Hit
TCCGTAGGTGAACCTGGGCTGTTCTCTTCTTAATTAGCTCTGGCCTGCCG7430.10282242719051211No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTGCATCGATGAAGAACGCAGC7420.10268403899779271No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATTGT7420.10268403899779271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)