FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722605
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG11975916.57323157188229No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN598138.277412971125303No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA430585.958718802111804No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC370805.131434186035247No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG363375.028611758844735No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC336774.660499166211139No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA279573.868918703856187No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG267993.7086651766871253No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG196792.723341244525017No Hit
GCTGCGTTCTTCCATCAGAGTCATGTGGGGTTTCAGAGTCAGCTCCCCTG169242.342081773583078No Hit
GCTGCGTTCTTCATCGATGCAAACATTCTCAATAAAATACTGGCAAACCG139571.9314840057846265No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG131801.823956380041655No Hit
GCTGCGTTCTTCATCGATGCCTCCAATCACAGCCAGGACTGCCATGAGGC120461.6670241694978585No Hit
GCTGCGTTCTTCATCGATGCCCTGCTGGCTGACATCAGGAAGGGCTTCCA87631.2126957328000776No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA74431.0300233184104732No Hit
GCTGCGTTCTTCATCGATGCCACGCAAACACCGCAGGTTCACCTACGGA72921.0091267013098442No Hit
GCTGCGTTCTTCAGATAGAAGCTCCTCACCAGGAAGTCCTCACACCAGAT58910.8152448433099688No Hit
GCTGCGTTCTTCATCGATTTGTGCTGCTATAACAATATACGACAGAGTGA58640.8115083621065451No Hit
GCTGCGTTCTTCATCGATGCCAAAGGTTTCAGGATTTCCTCCGCCCTCCC55910.7737283854941496No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA51370.7109001459995433No Hit
GCTGCGTTCTTCATCGATGCAAAAATTCTCAATAACATACTGGCAAATCA51300.7099314286505075No Hit
GCTGCGTTCTTCATCGATGCGAAAATACACAATAAAATACTGGCAAACCA51000.7057797828689256No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATATGCCAAACCAA40920.5662844846077733No Hit
GCTGCGTTCTTCATCGATGCACACATGGGGGCCACAGCAGGGGACCGCTC40100.5549366528047827No Hit
GCTGCGTTCTTCATCACACCATCCAGCACCCCCATCTCCATTCTATGCCT39150.54178977449644No Hit
GCTGCGTTCTTCATCGATGCCTGGTGGAAGTGGGCTTACTCTGCATCCTG38260.5294732253444137No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATATGGGCAAATTG36220.5012420340296566No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGTGGCAAGGATCTACG35420.49017097861210485No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG34090.471765348980425No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAGTACTGGCAAACCG33870.46872080874059824No Hit
GCTGCGTTCTTCATCGATGCTGTAAATGTAACACTAGAAGCACCTCGCCGGTTCCCCTACGGA32580.45086873187979604No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCA31620.4375834653787339No Hit
GCTGCGTTCTTCATCGATGCAAAAATCTTCAATAAAATACTGGCAAACGA28400.39302246732308804No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTACG28280.3913618090104552No Hit
GCTGCGTTCTTCATCGATGCTCACACGTGTACTGAGCAGTGAGAGGGGAA26260.363407394081137No Hit
GCTGCGTTCCCATCCTCATGATTCTGTCTGGTGCTTTCTGCACAGCATAG25200.3487382456528809No Hit
GCTGCGTTCTTCATCCAGCTCCAACCTAAGAGGTCCACCTGTGTGGTTACTGCTGCAGGTTCACCTACGGA20860.28867777001266254No Hit
GCTGCGTTCTTCATCGATGGAGCCATGGAGCTTCCCCTTGGTGATCTGGG19900.2753925035116004No Hit
GCTGCGTTCTTCCATCAGAGTCATGTTGGGTTTCAGAGTCAGCTCCCCTG18480.255741380145446No Hit
GCTGCGTTCTTCATCGATGCTGTAAATGTAACACTAGAAGCACCTCGCCGGTTCACCTACGGA16110.22294337847094886No Hit
GCTGCGTTCTTCATCGATGCCACTGTGCTTTCTCCCAGCCAGGCCCTACT15620.21616235702769843No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC13860.19180603510908448No Hit
GCTGCGTTCTTCATCGATGCCTCCAATCACAGCCATGACTGCCATGAGGC12800.1771368866808284No Hit
GCTGCGTTCTTCATCACAGACAATTGCTGAGAACTGACCCTGGGCTCACA11750.16260612644529168No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATACTGGCAAACTG11640.1610838563253783No Hit
GCTGCGTTCTTCATCGATGCCCTGCTTGCTGACATCAGGAAGGGCTTCCA11010.1523654001840563No Hit
GCTGCGTTCTTCATCGATGCTGTAAATGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA10950.15153507102773991No Hit
GCTGCGTTCTTCATCGATGCCACGCAAACACCGCATGTTCACCTACGGC9790.13548204067228986No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA8580.11873706935324277No Hit
GCTGCGTTCTTCATCGCACCATCCAGCACCCCCATCTCCATTCTATGCCT8530.11804512838964579No Hit
GCTGCGTTCTTCATCCCTCTGTGGAGCACATGGACTGCCCACACTCAGGG8500.1176299638114876No Hit
GCTGCGTTCTTCATCCCCCCATGGAAGAAGTGATCCAAGAAGTATTTTCA8210.11361670622262508No Hit
GCTGCGTTCTTCATCGATGCAACAAAAACATTATGGAATCTAAACTAAAG8090.11195604790999233No Hit
GCTGCGTTCTTCATCGAAAGTACTGACTTAGCAAGTGCTTCCTGTGCCAC7960.11015700140464015No Hit
GCTGCGTTCTTCCATCAGAGTCAGGTTGGGTTTCATAGTCAGCTCCCCTG7720.1068356847793746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)