FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816084
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGAGGCTCTACGCTTGATG11899414.58109704393175No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGATAGGGTCCTTCGGG9299411.395150499213317No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN688358.43479347714206No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC435495.336337925997814No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA423635.191009748996427No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATCAT387714.750858980203018No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGGCCGAGAGGCCT278363.410923385337784No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGACTGT225882.767852328926924No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC157431.929090632827013No Hit
TCCGTAGGTGAACCTGCCTTGCATCCTGGGGTGAAGCCAACTTGATCGTG155961.9110777812087971No Hit
TCCGTAGGTGAACCTGCGAATACCTCGATGTTGGGCCTCCCAGTTTCCAG149481.8316741904019682No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG145111.7781257811695854No Hit
TCCGTAGGTGAACCTGCGGCTTCCTGGGAGAGAGGAGAAAAGTCAAAGGC132271.6207890364227213No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACACCTCAAAATTCCTTA120471.4761960778547305No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC110441.3532920630719387No Hit
TCCGTAGGTGAACCTGCCCCATGGGTCCTTGCCTGCTACACAGACAGAAT99431.2183794805436696No Hit
TCCGTAGGTGAACCTGCATGAGGCAGCGGTCCCTCAGAGAGTCTGTTTCT92291.1308884869694786No Hit
TCCGTAGGTGAACCTGCGGACCCCAAGTACCATGAAGATGGGGATGGGAT71980.8820170472647424No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG71060.8707436979526617No Hit
TCCGTAGGTGAACCTGTAGGGATTGGGCAGCTCCGTGAGTGTCTCTGTCG69910.8566520113125609No Hit
TCCGTAGGTGAACCTGCGTGCCTGTGGCTCACTCCAGGGGTGTGTTGCGT69810.8554266472569001No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA66070.809598031575181No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACAAAAAATGAACTAATAAC62550.7664652168159161No Hit
TCCGTAGGTGAACCTGCGGCGTGTTTGCTCCCCTCTGAGCTGTGAGACCC53160.651403531989354No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGGTCAT52450.6427034471941614No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCAACTTGTTCGT51230.627754005715098No Hit
TCCGTAGGTGAACCTGCATCCCAGGGATTAAGCACACTTGATCTTGGTGG50220.6153778287529225No Hit
TCCGTAGGTGAACCTGCGGGAGCTGTACCTTAGCCACAATGGCATCGATGAAGAACGCAGC47490.581925390033379No Hit
TCCGTAGGTGAACCTGCCCCTGCCAGGGTTTCAGCAGGGCCAAGCTGGAA45090.5525166526975165No Hit
TCCGTAGGTGAACCTGCGGTGGCAATCGCAGAAGTGAAGCTCGGAGGATT43030.5272741531509011No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATCGT38480.4715200886183285No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCAACTTGATCGT38330.4696820425348371No Hit
TCCGTAGGTGAACCTGCCACCTGGCCCTTGAGCCTAGAATGGCATCAGGG32850.4025320922846178No Hit
TCCGTAGGTGAACCTGCAGCCTCAGAGCAGCAGCCCATAACCCAAGTGTC30140.36932472637620634No Hit
TCCGTAGGTGAACCTGCACGTCTGAGTCCAGGATGCCAGTGATGTTTCTT26500.32472147475014823No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGAGATAAAGCTGACTTGATCGT25180.3085466692154239No Hit
TCCGTAGGTGAACCTGGCAGGATTCCAACCCCCAGCTCAATAATACTGTA23060.28256895123541204No Hit
TCCGTAGGTGAACCTGCGGCACGGAGGAGACGCACTAGAACCTTCCAGTG22780.27913793187956143No Hit
TCCGTAGGTGAACCTGCTGAGGCCAGGAGAAGGAGGTCATAGGGCAGCAG21620.26492370883389454No Hit
TCCGTAGGTGAACCTGCACCGCATCGATGAAGAACGCAGC20590.2523024590605869No Hit
TCCGTAGGTGAACCTGCGGCTCTCTGGGCATCGATGAAGAACGCAGC19180.23502482587576767No Hit
TCCGTAGGTGAACCTGCGGGGACAATGGCCCATGACATTTCAGTGGCTGC15790.19348498438886194No Hit
TCCGTAGGTGAACCTGCCTTGCACCCCAGGGATGAAGCTGACTTGATCAC14390.17632988760960883No Hit
TCCGTAGGTGAACCTGCTTCTTGGAAGGCAAGGACTCAGTCCTGGCAGGA14330.17559466917621225No Hit
TCCGTAGGTGAACCTGCTCTTTGGTAGGGAGCATCGATGAAGAACGCAGC14320.17547213277064616No Hit
TCCGTAGGTGAACCTGCTGAGGGATCATTAACTGAGAAGAGCGAAGCCGC13590.16652697516432133No Hit
TCCGTAGGTGAACCTGCGTGCTCCTGTACTGGGTGCATATACATTTAGGA13050.15991000926375226No Hit
TCCGTAGGTGAACCTGCGGCAGGAAGCACAGGACAGTGGCACAGGAAGCA12800.15684659912459992No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGGCCGCGAGGCCT12410.15206767930752227No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG11550.1415295484288382No Hit
TCCGTAGGTGAACCTGCGGCCCCGGGCTGTCTTCCGCAGCTGGAAGCCCT10320.1264575705442087No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA10230.12535474289411386No Hit
TCCGTAGGTGAACCTGCCGCCCGCCGCCAGAGGCCGAGGCCAGACGATCT8670.10623906362580322No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGAAGAAGCCGACTTGATCAT8390.10280804426995262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)