FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816084
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG15005618.387322873625756No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT10215412.517583974198734No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN679658.328186804299557No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCA300623.683689424127908No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG289973.553188152200019No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT283623.475377534665549No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAAAAAAATACTACCAAACTG208482.5546389832419214No Hit
GCTGCGTTCTTCATCGATTTGTGCTGCTATAACAATATACGACAGAGTGA137541.6853657221560525No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATATGCCAAACCAA114941.408433445576681No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA109001.3356468206704213No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC99331.2171541164880086No Hit
GCTGCGTTCTTCATCGATGCTCACACGTGTACTGAGCAGTGAGAGGGGAA95501.1702226731561949No Hit
GCTGCGTTCTTCATCGATGCATTAATCCATGGCTAAAACATGACTCATCG93191.141916763470427No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG91221.1177770915739067No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG79690.9764926159562006No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGTGGCAAGGATCTACG65740.8055543301914998No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG60020.7354635062076943No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCATCGTTGAAAATTT59250.7260282029791051No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCATTGCTGAAAGTGT57970.7103435430666452No Hit
GCTGCGTTCTTCATCGATGCCTCCAATCACAGCCAGGACTGCCATGAGGC57570.7054420868440014No Hit
GCTGCGTTCTTCATCGTTGTGGGAGCCAAGACATCCATTGCTGAAAGTGT57380.7031138951382455No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTACG53300.6531190416672794No Hit
GCTGCGTTCTTCATCGATGCTGCTCAGCATCCCATCCCCATCTTCATGGT49730.6093735448801839No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAGTACTGGCAAACCG48810.5981001955681033No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC47800.5857240186059278No Hit
GCTGCGTTCTTCATCGATGCAAAAGTCCTCAACAAAATACTGGCAAACCA47160.5778816886496978No Hit
GCTGCGTTCTTCATCGATGCAAACATTCTCAATAAAATACTGGCAAACCG46070.5645252204429937No Hit
GCTGCGTTCTTCATCGATCACCAGCACCGAGACCATCTCACACAGTACTC43620.5345038010793007No Hit
GCTGCGTTCTTCATCACAGACAATTGCTGAGAACTGACCCTGGGCTCACA37040.45387484621681096No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAAACA34930.42801966464236524No Hit
GCTGCGTTCTTCATCGATGCCTGGTGGAAGTGGGCTTACTCTGCATCCTG34610.4240984996642503No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG32700.4006940462011264No Hit
GCTGCGTTCTTCATCGATGCCATTGTGGCTAAGGTACAGCTCCCGCAGGTTCACCTACGGA29560.36221761485337295No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATATGCCAAACCCA28790.3527823116247837No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT23460.2874704074580558No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATATGGGCAAATTG23390.28661265261909313No Hit
GCTGCGTTCTTCATCGATGCTGTAAATGTAACACTAGAAGCACCTCGCCGGTTCCCCTACGGA22860.2801182231240902No Hit
GCTGCGTTCTTCATCGATGGAGCCATGGAGCTTCCCCTTGGTGATCTGGG22290.2731336480068228No Hit
GCTGCGTTCTTCATCGATGCAACAAAAACATTATGGAATCTAAACTAAAG19640.24066150053180801No Hit
GCTGCGTTCTTCATCGATGCACCTCAGGAAAGGATAAGCAAGACGTGTGA19240.23576004430916425No Hit
GCTGCGTTCTTCATCATGCGCTGTCCTCTCATCAAGTCTTTTTCTTTCTT18710.22926561481416127No Hit
GCTGCGTTCTTCATCGATGCCCAGAGAGCCGCAGGTTCACCTACGGA18040.22105567564123302No Hit
GCTGCGTTCTTCATCGATGCAGACACCGGGCTTTTGCACTGGAAGGTTCT17780.21786972909651459No Hit
GCTGCGTTCTTCATCGATGCCACTGTGCTTTCTCCCAGCCAGGCCCTACT16070.19691600374471255No Hit
GCTGAGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG14550.1782904700986663No Hit
GCTGCGTTCTTCATCGATGCGGTGCAGGTTCACCTACGGA14120.17302140465932428No Hit
GCTGCGTTCTTCATCGATGCCAAAGGTTTCAGGATTTCCTCCGCCCTCCC13640.1671396571921518No Hit
GCTGCGTTCTTCATCGATGCTCCCTACCAAAGAGCAGGTTCACCTACGGA13560.16615936594762304No Hit
GCTGCGTTCTTCATCGATGCAACACTCCTCTGTAAAATACTAGCAAACCG12990.1591747908303557No Hit
GCTGCGTTCTTCATCGATGCTGTAAATGTAACACTAGAAGCACCTCGCCGGTTCACCTACGGA11160.13675062861176057No Hit
GCTGCGTTCTTCATCGATGCTAGGAAGAAACTGCATCAACTAACGAGCAA10990.13466750971713695No Hit
GCTGAGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT10880.13331960925590994No Hit
GCTGCGTTCTTCATCGATGCTCACACTTGTACTGATCAGTGAGAGGGGCA10070.12339416040505634No Hit
GCTGCGTTCTTCATCGATGCCTGGTGTAAGTGGGCTTACTCTGCATCCTG9950.12192372353826322No Hit
GCTGCGTTCTTCATCGATGCCTCCAATCACAGCCATGACTGCCATGAGGC9890.12118850510486666No Hit
GCTGCGTTCTTCATCGAAAGTACTGACTTAGCAAGTGCTTCCTGTGCCAC9230.11310110233750446No Hit
GCTGCGTTCTTCATCGATGCTCACACTTGTACTGAGCAGTGAGAGGGGCA9160.11224334749854181No Hit
GCTGCGTTCTTCATCGATGCTGCTCATCATCCCATCCCCATCTTCATGGT8450.10354326270334917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)