FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813137
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG16935820.82773259610619No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA10804213.287059868140302No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG508226.250115294224711No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT504876.208916824594134No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT460415.662145493317854No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC415615.111192824825337No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT408615.025106470373381No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG256403.153220183068782No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN200392.4644063669467755No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCA120201.4782256864464414No Hit
GCTGCGTTCTTCATCGATGCGCACGATCGCGCAAAGTCCCGTTCGAAGAC99481.2234100772686523No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA96091.1817196856126335No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG93491.14974475395905No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAAACA84861.0436125769704243No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATATGGGCAAATTG69620.8561902852778805No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG64240.7900267728562345No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG59290.7291514222080658No Hit
GCTGCGTTCTTCATCGATGCCCTGCTGGCTGACATCAGGAAGGGCTTCCA48760.5996529490110523No Hit
GCTGCGTTCTTCATCGATGCTCCAGATGGTTCACCTACGGA45070.5542731421642355No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA42390.5213143664597725No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGATATCCATTGCTAAGAGTTG41170.5063107446838602No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA38840.4776562867019949No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTG35580.43756464162865544No Hit
GCTGCGTTCTTCATCGATGCTGGTTGGAGTAGCAATGTGCTCAATATGTGAGCAGGTTCACCTACGGA30330.3729998757896886No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG26230.3225778681821145No Hit
GCTGCGTTCTTCATCGATGCAAACATTCTCAATAAAATACTGGCAAACCG25560.3143381742559987No Hit
GCTGCGTTCTTCATCGATGCGCACGATCGCGCAAAGTCCCGTTCGAAGCC15540.19111170688334192No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG14800.1820111494127066No Hit
GCTGAGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG14310.17598510460106967No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG13900.17094290384031227No Hit
GCTGCGTTCTTCATCGATGCAACAAAAACATTATGGAATCTAAACTAAAG12010.1476995881382842No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATATGCCAAACCAA11850.14573190003652522No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGTGGCAAGGATCTACG11610.14278036788388673No Hit
GATGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG10630.1307282782606129No Hit
GCTGCGTTCTTCATCGATGCGCACGATCGCGCAAATTCCCGTTCGAAGCC9900.12175070129633753No Hit
GCTGAGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA9460.11633955901650031No Hit
GCTGCGTTCTTCATCGATGCCGCAGGTTCACCTACGGA9430.11597061749742048No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTACG8940.10994457268578357No Hit
GCTGCGTTCTTCATCGATGCCCTGCTTGCTGACATCAGGAAGGGCTTCCA8630.10613217698862552No Hit
GCTGCGTTCTTCATCGATGCAGGGTCACCTACGGA8300.10207382027874762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)