FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001565396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736722
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGAGGCTCTACGCTTGATG11372515.436623312457073No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG8115311.0154169415329No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACC7694710.444509597921604No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN460576.251611869877648No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGGCTAA450636.116689877592905No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC315574.283433914013698No Hit
TCCGTAGGTGAACCTGCGGAAGGGTCATTAGTGATTTGCCTTCGGGCTAA240643.266360988269659No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC197752.6841875225661784No Hit
TCCGTAGGTGAACCTGCATCCCAGGGATTAAGCACACTTGATCTTGGTGG190342.583606842200993No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATCAT181572.4645660099739115No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC174512.3687361039849497No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA133071.8062444178401078No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATCGT128851.7489636525039296No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATAAATAGGGTGTCCA90341.2262427347086147No Hit
TCCGTAGGTGAACCTGCCACCTGGCCCTTGAGCCTAGAATGGCATCAGGG86991.18077103710762No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCAACTTGATCAT76171.0339042406769445No Hit
TCCGTAGGTGAACCTGCCCCCCAGCACAGGGCCATGCTGCACCAAGGTCA73580.9987485102928919No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTGCATCGATGAAGAACGCAGC68110.924500693613059No Hit
TCCGTAGGTGAACCTGTAGGGATTGGGCAGCTCCGTGAGTGTCTCTGTCG67500.9162207725573555No Hit
TCCGTAGGTGAACCTGCCCCTGCCAGGGTTTCAGCAGGGCCAAGCTGGAA67120.9110627889488844No Hit
TCCGTAGGTGAACCTGCGAATACCTCGATGTTGGGCCTCCCAGTTTCCAG66460.9021041858394347No Hit
TCCGTAGGTGAACCTGCGGAATCTGATGATAAAGCTTTAACATCGTAAAT49310.6693162414044918No Hit
TCCGTAGGTGAACCTGCATGAGGCAGCGGTCCCTCAGAGAGTCTGTTTCT48230.6546567090435741No Hit
TCCGTAGGTGAACCTGGTCTCCGAGCACATCTGGTGTGAGGACTTCCTGG46890.6364680300031763No Hit
TCCGTAGGTGAACCTGGGCTGTTCTCTTCTTAATTAGCTCTGGCCTGCCG36250.4920444889659871No Hit
TCCGTAGGTGAACCTGCGGCAGGAAGCACAGGACAGTGGCACAGGAAGCA31250.4241762835913683No Hit
TCCGTAGGTGAACCTGCCTTGCATCCTGGGGTGAAGCCAACTTGATCGTG28630.3886133439750679No Hit
TCCGTAGGTGAACCTGCACCGCATCGATGAAGAACGCAGC28130.38182652343760604No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG26120.35454350487700925No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTACTTGCACC24090.32698901349491394No Hit
TCCGTAGGTGAACCTGCAGCAGTAACCACACAGGTGGACCTCTTAGGTTGGAGCTGGATGAAGAACGCAGC20510.2783953784466868No Hit
TCCGTAGGTGAACCTGCGGGAGCTGTACCTTAGCCACAATGGCATCGATGAAGAACGCAGC19240.26115685428153357No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCCCTTGATCAT18940.2570847619590565No Hit
TCCGTAGGTGAACCTGCGGCCCCGGGCTGTCTTCCGCAGCTGGAAGCCCT17140.23265220802419365No Hit
TCCGTAGGTGAACCTGCTGAGGCCAGGAGAAGGAGGTCATAGGGCAGCAG16900.22939453416621194No Hit
TCCGTAGGTGAACCTGCTACCTGAAAGACAAAGTATAAAGAATGAAAAGA16730.2270870151834749No Hit
TCCGTAGGTGAACCTGCCCCCATAAGCACCCTCCTTGTGTCCCCAGAAGC16050.21785693925252675No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGAGATAAAGCTGACTTGATCGT15700.21310616487630343No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATTGT15520.21066290948281713No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATTGT14010.19016671145968223No Hit
TCCGTAGGTGAACCTGCTGCCAGCCAGGCCTGCAGCGCCCGGGGCGATTT13260.17998648065348938No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC12420.1685846221505534No Hit
TCCGTAGGTGAACCTGCGTCGATGAAGAACGCAGC12320.16722725804306102No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG11330.15378935337888647No Hit
TCCGTAGGTGAACCTGCAGGTGCAAAGGCCCGGGCAGGTGGAAGTGGTGC10810.1467310600199261No Hit
TCCGTAGGTGAACCTGCCCTGAAAGAATATTATGTAAATCTTTCTGTCAC10300.139808503071715No Hit
TCCGTAGGTGAACCTGCCTTGTATCCCAGGGATGAAGCCGACTTGATTGT9420.12786369892578206No Hit
TCCGTAGGTGAACCTGCGGGTGCAAAGGCCCGGGCAGGTGGAAGTGGTGC9120.12379160660330492No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGTGCTAA9050.12284145172806024No Hit
TCCGTAGGTGAACCTGCGCTGTGTAGCCCCCATGCTTCAAAGAACGCAGC9050.12284145172806024No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGGTCAT8720.1183621501733354No Hit
TCCGTAGGTGAACCACGCCTCTATGTTTGCGTCGCTTTCGGAGCCACGCC8160.11076091117137808No Hit
TCCGTAGGTGAACCTGCCCCCATGATTCAGTTAACTCCCACTGTGTCCCT8000.10858912859939027No Hit
TCCGTAGGTGAACCTGCGGCTTCCTGGGAGAGAGGAGAAAAGTCAAAGGC7780.10560292756290704No Hit
TCCGTAGGTGAACCTGGCAGGATTCCAACCCCCAGCTCAATAATACTGTA7620.10343114499091924No Hit
TCCGTAGGTGAACCTGTTCTCCGAGCACATCTGGTGTGAGGACTTCCTGG7500.10180230806192839No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)