FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736722
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG10713614.542256102030345No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT7678210.422113090147981No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA686899.3235983179544No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA620058.416336148506492No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN458096.217949240011836No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG245763.3358580305732697No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC184662.506508560895426No Hit
GCTGCGTTCTTCATCGATGCAAACATTCTCAATAAAATACTGGCAAACCG181942.469588257171633No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA179192.432260744215593No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG154782.100928165576703No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA141501.9206702121017154No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG124671.692225832810748No Hit
GCTGCGTTCTTCATCGATGCAAAAGTCCTCAATAAAATACCGGCAAACTG114711.5570323677045073No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT91501.2419881583555261No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATGCTGGCAAACCA70570.9578918506573714No Hit
GCTGCGTTCTTCATCGATGCCCAGGTTGTTGTTGATGATTTTCAGAATGT68840.9344094515977532No Hit
GCTGCGTTCTTCATCGATGCACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA66820.9069906966264073No Hit
GCTGCGTTCTTCATCGATGGAGCCATGGAGCTTCCCCTTGGTGATCTGGG66780.9064477509834103No Hit
GCTGCGTTCTTCATCGATTTGTGCTGCTATAACAATATACGACAGAGTGA63360.8600258985071709No Hit
GCTGCGTTCTTCATCGATGCCTCCAATCACAGCCAGGACTGCCATGAGGC59990.8142827280846778No Hit
GCTGCGTTCTTCATCGATGCCTGGTGGAAGTGGGCTTACTCTGCATCCTG56080.7612097914817259No Hit
GCTGCGTTCTTATCAGACGATGGCAAATTAATGATTGATGATCAACAATA47220.6409473315579011No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG44520.6042985006556069No Hit
GCTGCGTTCTTCAGATAGAAGCTCCTCACCAGGAAGTCCTCACACCAGAT43370.5886888134194446No Hit
GCTGCGTTCTTCATCACAGAGAGTTCATTTGCTCTCCTCGGCAGGCCAGA32740.4444010087930047No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGTGGCAAGGATCTACG30980.42051140050113883No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCA27940.37924753163337055No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTACG25770.3497927305007859No Hit
GCTGCGTTCTTCATCGAAAGTACTGACTTAGCAAGTGCTTCCTGTGCCAC25220.3423272279095778No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATATGCCAAACCAA23470.3185733560284612No Hit
GCTGCGTTCTTCATCGATGCGGTGCAGGTTCACCTACGGA22690.3079859159900207No Hit
GCTGCGTTCTTCCACACTGCTCCCAGCCCTGGCTTCTCGCCTGTAAACTG19960.27092987585547873No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTG17330.2352311998284292No Hit
GCTGCGTTCTTCATCCAGCTCCAACCTAAGAGGTCCACCTGTGTGGTTACTGCTGCAGGTTCACCTACGGA17210.2336023628994383No Hit
GCTGCGTTCTTCATCGATGCTTAGTGTAGTTAGTCTTCTTATTTTTAGCC15950.21649957514503437No Hit
GCTGCGTTCTTCATCGATGCTGTAAATGTAACACTAGAAGCACCTCGCCGGTTCCCCTACGGA15130.20536918946359684No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATATGGGCAAATTG14880.2019757791948659No Hit
GCTGCGTTCTTCATCGATGCCATTGTGGCTAAGGTACAGCTCCCGCAGGTTCACCTACGGA14580.19790368687238874No Hit
GCTGCGTTCTTCATCGATGCCCTGCTGGCTGACATCAGGAAGGGCTTCCA14460.19627484994339792No Hit
GCTGCGTTCTTCATCGATGCCACTGTGCTTTCTCCCAGCCAGGCCCTACT13940.18921655658443753No Hit
GCTGCGTTCTTCATCGATGGAGCCATTGAGCTTCCCCTTGGTGATCTGGG13610.1847372550297127No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAAAAAAATACTACCAAACTG13400.1818867904039787No Hit
GCTGCGTTCTTCATCATCATAATGGCCAGGCGCTTGGCCTCACTCTCCTC13210.1793077985997432No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG11320.15365361696813723No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA11030.14971726105640934No Hit
GCTGCGTTCTTCATCGACGCAGGTTCACCTACGGA10940.1484956333596662No Hit
GCTGCGTTCTTCATCGATGCCCTTCCCGGAGCTGGGCGGGGTGGCGGCGC10500.14252323128669972No Hit
GCTGCGTTCTTCATCGATGCCTGGTGTAAGTGGGCTTACTCTGCATCCTG9550.12962827226552215No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC9300.1262348619967912No Hit
GCTGCGTTCTTCATCGATGCTATTCCTTTGGGGAACAAAATTTCCACTTA9160.12433455224630187No Hit
GCTGCGTTCTTCATCGATGCGAAAATACACAATAAAATACTGGCAAACCA9090.12338439737105719No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAGTACTGGCAAACCG8250.1119825388681212No Hit
GCTGCGTTCTTCATCGATGCTGTAAATGTAACACTAGAAGCACCTCGCCGGTTCACCTACGGA7880.10696029167039942No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAGTAAAATACTGGCAAACCG7780.10560292756290704No Hit
GCTGCGTTCTTTGAAGCATGGGGGCTACACAGCGCAGGTTCACCTACGGA7420.10071641677593447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)