FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001565398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720815
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGAGGCTCTACGCTTGATG23656232.81868440584616No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN551357.648980667716404No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC503856.990004370053342No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG261813.632138620866658No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC227753.1596179324792075No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC178262.4730339962403667No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGAGATAAAGCTGACTTGATCGT164932.2881044373382906No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATCAT161322.238022238715898No Hit
TCCGTAGGTGAACCTGCATCCCAGGGATTAAGCACACTTGATCTTGGTGG122781.7033496805699104No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATCGT121861.6905863501730682No Hit
TCCGTAGGTGAACCTGTAGGGATTGGGCAGCTCCGTGAGTGTCTCTGTCG117821.634538681908673No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG105621.4652858222983705No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG90701.2582978989060993No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA75361.0454832377239651No Hit
TCCGTAGGTGAACCTGCGGAATCTGATGATAAAGCTTTAACATCGTAAAT62260.8637445114210998No Hit
TCCGTAGGTGAACCTGCGTGCCTGTGGCTCACTCCAGGGGTGTGTTGCGT62180.862634656603983No Hit
TCCGTAGGTGAACCTGCGGCATCGATGAAGAACGCAGC58500.8115813350166132No Hit
TCCGTAGGTGAACCTGCCTTGTATCCCAGGGATGAAGCCGACTTGATTGT58260.8082517705652629No Hit
TCCGTAGGTGAACCTGCCTTGTATCCCAGGGATTAAGCCAACTTGATTTT58250.8081130387131233No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACC53410.7409668222775608No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATGTGAAACCTTCCGACCCCTCTC49920.6925494058808432No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG47560.6598086887758995No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGGTCAT45890.6366404694685877No Hit
TCCGTAGGTGAACCTGCGTTGCATCCCAGCGATGAGGCTGACTTGATCAT44980.6240158709238848No Hit
TCCGTAGGTGAACCTGCGGTGGCAATCGCAGAAGTGAAGCTCGGAGGATT43930.6094490264492276No Hit
TCCGTAGGTGAACCTGCGGACACACAGCTGCCACTCTGGGGACCCCGACC42330.5872519301068929No Hit
TCCGTAGGTGAACCTGCGGGGAGGAGGTTGCAGTGAGCCGAGATCGTGCC41230.5719914263715378No Hit
TCCGTAGGTGAACCTGTACGAGCAGTTCCACCGCGTGTCCAACCTGTTCT39030.5414704189008276No Hit
TCCGTAGGTGAACCTGGGCTGTTCTCTTCTTAATTAGCTCTGGCCTGCCG32680.4533756927921866No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATAAAGCCAACTTGATGGT31200.43284337867552697No Hit
TCCGTAGGTGAACCTGCGGCTTCCTGGGAGAGAGGAGAAAAGTCAAAGGC29650.41133994159389026No Hit
TCCGTAGGTGAACCTGCGGGAGCTGTACCTTAGCCACAATGGCATCGATGAAGAACGCAGC28690.3980216837884894No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATTGT25930.35973169259796206No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTGCATCGATGAAGAACGCAGC25570.3547373459209367No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG25530.3541824185123783No Hit
TCCGTAGGTGAACCTGCGAATACCTCGATGTTGGGCCTCCCAGTTTCCAG24630.34169655181981506No Hit
TCCGTAGGTGAACCTGCTTCTGGAGGCACAAGTAGTGTAGCTCCTCTTAGGCCCTTTCGATGAAGAACGCAGC24560.3407254288548379No Hit
TCCGTAGGTGAACCTGCATGAGGCAGCGGTCCCTCAGAGAGTCTGTTTCT24350.3378120599599065No Hit
TCCGTAGGTGAACCTGCGGTCGTCCTGTTGTTCTTGGAGGCATCGATGAAGAACGCAGC23320.32352267918952854No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCAACTTGATCGT21090.2925854761623995No Hit
TCCGTAGGTGAACCTGGCAGGATTCCAACCCCCAGCTCAATAATACTGTA20800.28856225245035133No Hit
TCCGTAGGTGAACCTGCGGCCCCGGGCTGTCTTCCGCAGCTGGAAGCCCT18000.2497173338512656No Hit
TCCGTAGGTGAACCTGCTCACACACCCAGTACGTCATTATTGCAGCCAGCATCGATGAAGAACGCAGC16980.2355666849330272No Hit
TCCGTAGGTGAACCTGCCTTGCATCCTGGGGTGAAGCCAACTTGATCGTG14430.20019006263743122No Hit
TCCGTAGGTGAACCCGAGGAAGGCCCCCCAGGCCCTGTGGGCATCGGTGA14240.197554157446779No Hit
TCCGTAGGTGAACCTGCGGATGTAAAGAACGCAGC14200.19699923003822065No Hit
TCCGTAGGTGAACCTGCGGGTCATCATGCTAGGAGGGAGGTTGTTCTGCA12150.16855920034960428No Hit
TCCGTAGGTGAACCTGCAGCAGTAACCACACAGGTGGACCTCTTAGGTTGGAGCTGGATGAAGAACGCAGC11250.15607333365704099No Hit
TCCGTAGGTGAACCTGCTGAATGATGCATAATGGCATCGATGAAGAACGCAGC11220.1556571381006222No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATTGT10830.15024659586717812No Hit
TCCGTAGGTGAACCTGCGCATCGATGAAGAACGCAGC10680.14816561808508424No Hit
TCCGTAGGTGAACCTGCGGGCCTGGAGACCACCCTGGACCAGGCTTCAGT9040.12541359433419114No Hit
TCCGTAGGTGAACCTACGCATCGATGAAGAACGCAGC8800.12208402988284095No Hit
TCCGTAGGTGAACCTGCGGTGGCAATCACAGAAGTGAAGCTCGGAGGATT8690.12055797950930544No Hit
TCCGTAGGTGAACCTGCCCCCCAGCACAGGGCCATGCTGCACCAAGGTCA8570.11889319728363033No Hit
TCCGTAGGTGAACCTGCGGCAGGAAGCACAGGACAGTGGCACAGGAAGCA8450.11722841505795523No Hit
TCCGTAGGTGAACCTGCACCGCATCGATGAAGAACGCAGC8270.11473124171944256No Hit
TCCGTAGGTGAACCTGCCAAGTAAGTCCCCACTTGGGAGGCATCGATGAAGAACGCAGC8250.1144537780151634No Hit
TCCGTAGGTGAACCATGCTGAATGATGCATAATGGCATCGATGAAGAACGCAGC8050.11167914097237154No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGAGAGGAAGCCAACTTGATGGT7320.10155171576618134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)