FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720815
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG22287830.920277741167983No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN547177.590990753522055No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG392385.4435604142533105No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA249763.4649667390384495No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG199862.7726947968618854No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA185922.5793025949792945No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC165282.2929600521631763No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATATGGGCAAATTG157422.183916816381457No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT141861.9680500544522521No Hit
GCTGCGTTCTTCATCGATGCAAACATTCTCAATAAAATACTGGCAAACCG117411.6288506759709493No Hit
GCTGCGTTCTTCATCGATGCCTCCAATCACAGCCAGGACTGCCATGAGGC105281.4605689393256245No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT99581.3814917836060572No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG71420.9908228879809661No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG70830.9826377087047301No Hit
GCTGCGTTCTTATCAGACGATGGCAAATTAATGATTGATGATCAACAATA60420.8382178506274148No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTG57520.7979856135069331No Hit
GCTGCGTTCTTCATCGATGCGAAAATACACAATAAAATACTGGCAAACCA55790.7739850030867838No Hit
GCTGCGTTCTTCATCGATGCAAAAATTCTCAATAACATACTGGCAAATCA55180.7655223601062686No Hit
GCTGCGTTCTTCATCGATCACCAGCACCGAGACCATCTCACACAGTACTC52310.7257063185422057No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC49450.6860290088302824No Hit
GCTGCGTTCTTCATCGATGCCGCAGGTTCACCTACGGA48920.678676220666884No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGTGGCAAGGATCTACG48500.6728494828770211No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAGTACTGGCAAACCG43720.6065356575542962No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATACTGGCAAACCG42130.584477293064101No Hit
GCTGCGTTCTTCATCCTGCAGTTGGCTCAGAGCTGCAGCTGACTGTGGGT39670.5503492574377614No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTACG39130.5428577374222234No Hit
GCTGCGTTCCTCTCCACCTCCTGTAGCTCCCGAGGGCCACTCCAGCCCCC34230.47487912987382336No Hit
GCTGCGTTCTTCATCGATGCTGCCAGGTGGCTAGGGGGGACTTCCTGGAG31210.4329821105276666No Hit
GCTGCGTTCTTCATCACAGAGAGTTCATTTGCTCTCCTCGGCAGGCCAGA28500.3953857785978372No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACAG27710.38442596227880943No Hit
GCTGCGTTCTTCATCGATGCACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA25290.3508528540610281No Hit
GCTGCGTTCTTCATCGATGCTCACACGTGTACTGAGCAGTGAGAGGGGAA24140.33489869106497505No Hit
GCTGCGTTCTTCATCGATTTGTGCTGCTATAACAATATACGACAGAGTGA23660.3282395621622746No Hit
GCTGCGTTCTTCATCGATGCCATTGTGGCTAAGGTACAGCTCCCGCAGGTTCACCTACGGA21780.30215797396003136No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG21710.3011868509950542No Hit
GCTGCGTTCTTCATCGATGCAACAAAAACATTATGGAATCTAAACTAAAG20730.2875911294853742No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC20210.28037707317411537No Hit
GCTGCGTTCTTCATCGAAAGGGCCTAAGAGGAGCTACACTACTTGTGCCTCCAGAAGCAGGTTCACCTACGGA20150.27954468206127786No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAAACA20080.2785735590963007No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCA19980.2771862405749048No Hit
GCTGCGTTCTTCATCGATGCTGTAAATGTAACACTAGAAGCACCTCGCCGGTTCCCCTACGGA18930.26261939610024765No Hit
GCTGCGTTCTTCATCGATGCCTCCAAGAACAACAGGACGACCGCAGGTTCACCTACGGA18880.26192573683954967No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAGTAAAATACTGACAAACCA16550.22960121529102476No Hit
GCTGCGTTCTTCATCGATGCTGGCTGCAATAATGACGTACTGGGTGTGTGAGCAGGTTCACCTACGGA15640.21697661674632188No Hit
GCTGCGTTCTTCATCGATGCCCTGCTGGCTGACATCAGGAAGGGCTTCCA15020.2083752419136672No Hit
GCTGCGTTCTTTACATCCGCAGGTTCACCTACGGA11920.16536836775039365No Hit
GCTGCGTTCTTCATCGATGCCCACAGGGCCTGGGGGGCCTTCCTCGGGTT11830.1641197810811373No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATATGCCAAACCAA11820.16398104922899773No Hit
GCTGAGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG11230.1557958699527618No Hit
GCTGCGTTCTTCATCGATGCCTCCAATCACAGCCATGACTGCCATGAGGC10940.15177264624071363No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAAAAAAATACTACCAAACTG10300.14289380770377974No Hit
GCTGCGTTCTTCATCGATGGGGAGGGCAGAGAAGGGAAGAGGGAGAAGCT10250.1422001484430818No Hit
GCTGCGTTCTTCATCGATGCTGTAAATGTAACACTAGAAGCACCTCGCCGGTTCACCTACGGA9830.13637341065321892No Hit
GATGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG9460.13124033212405403No Hit
GCTGCGTTCTTCATCGATGCCATTATGCATCATTCAGCAGGTTCACCTACGGA9120.1265234491513079No Hit
GCTGCGTTCTTCATCCAGCTCCAACCTAAGAGGTCCACCTGTGTGGTTACTGCTGCAGGTTCACCTACGGA9010.12499739877777238No Hit
GCTGCGTTCTTCATCGATGCGCAGGTTCACCTACGGA8870.1230551528478181No Hit
GCTGCGTTCTTCATCGATGCTGCAGGTCAGCCTGGCCAGCCTTGCTGTGG8510.11806080617079279No Hit
GCTGCGTTCTTCATCGATGCGTAGGTTCACCTACGGA8180.11348265505018625No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG8150.11306645949376747No Hit
GCTGCGTTCTTCATCGATGCCCAGGTTGTTGTTGATGATTTTCAGAATGT7950.11029182245097563No Hit
GCTGCGTTCTTCATCGATGCCAAAGGTTTCAGGATTTCCTCCGCCCTCCC7870.1091819676338589No Hit
GCTGCGTTCTTCATCGATGCGAGAGCGAAGAGATCCGTTGTTGAAAGTTG7770.10779464911246296No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA7580.10515874392181072No Hit
GCTGCGTTCTTCATCGATGCCATTATGCATCATTCAGCATGGTTCACCTACGGA7440.10321649799185643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)