FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001565401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677391
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA10118714.93775382312431No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN557398.228482516006265No Hit
GCTGCGTTCTTCATCGATGTGGCATCTGTGGAGCCCACAGATGCAAGTGC529347.814393754862406No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG454426.708385555757309No Hit
GCTGCGTTCTTCATCGATTTGTGCTGCTATAACAATATACGACAGAGTGA387195.715901156053151No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG247963.660515123466358No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC213893.1575559757953675No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA206963.055251693630414No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG174372.5741410795242334No Hit
GCTGCGTTCTTCATCGATGCTGCCAGGTGGCTAGGGGGGACTTCCTGGAG157752.328787952600492No Hit
GCTGCGTTCTTCATCGATGCGCCGGATGGTGTCTTCATCCACCTCCACGC153842.271066488925894No Hit
GCTGCGTTCTTCATCGATGCAAACATTCTCAATAAAATACTGGCAAACCG152212.247003576959245No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA120091.7728313485121592No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG77631.1460146355649838No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG76201.1249042281341204No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATACTGGCAAACCG64610.9538065902853743No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCA60010.8858989859623172No Hit
GCTGCGTTCTTCATCGATGCCTCCAATCACAGCCAGGACTGCCATGAGGC53120.7841852047045208No Hit
GCTGCGTTCTTCATCGATGCGAAAATACACAATAAAATACTGGCAAACCA49010.7235112364941371No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTG47670.7037294561043769No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAAACA45280.6684470269017451No Hit
GCTGCGTTCTTCATCATGCATCAGTATGGGTTCAGTGTGGCTGAGTCTGC44470.6564893835318154No Hit
GCTGCGTTCTTCATCGATGCTCACACGTGTACTGAGCAGTGAGAGGGGAA44190.6523558771817164No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCAAAAGTTG40130.592420035105279No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC39740.586662651260498No Hit
GCTGCGTTCTTCATCGATGCCAATGGGCAGGTTCACCTACGGA39330.5806100169621385No Hit
GCTGCGTTCTTCATCGATGCCGCAGGTTCACCTACGGA37630.5555137284079653No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGTGGCAAGGATCTACG37400.5521183481918124No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA34910.5153596667212881No Hit
GCTGCGTTCTTCATCGATGCCCACAGGGCCTGGGGGGCCTTCCTCGGGTT34590.5106356594640318No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACAG33850.49971139268162695No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTACG30960.4570477021395324No Hit
GCTGCGTTCTTCATCGATGCACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA27180.40124536641319414No Hit
GCTGCGTTCTTCATCGATGCAAAAGTCCTCAACAAAATACTGGCAAACCA26230.38722096986821497No Hit
GCTGCGTTCTTCATCAACGCAGAAGGCCAGTGATTCCCGCACCCGCCGAC22580.333337762090137No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATATGCCAAACCAA22560.33304251163655846No Hit
GCTGCGTTCTTCATCGTTGTGGGAACCAAGACATCCGTTGCTGAAGGTGT22240.3283185043793024No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATATGGGCAAATTG21420.3162132357825835No Hit
GCTGCGTTCTTCATCGATGCGGTGCAGGTTCACCTACGGA19820.29259319949630275No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAGTAAAATATGCCAAACCAA18950.27974980476563754No Hit
GCTGCGTTCTTATCAGACGATGGCAAATTAATGATTGATGATCAACAATA17640.26041090005624523No Hit
GCTGCGTTCTTCATCGATGCAACAAAAACATTATGGAATCTAAACTAAAG17490.2581965216544064No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG16070.23723373945033224No Hit
GCTGCGTTCTTCATCACAGAGAGTTCATTTGCTCTCCTCGGCAGGCCAGA15850.2339859844609686No Hit
GCTGCGTTCTTCATCGATGCCATTGTGGCTAAGGTACAGCTCCCGCAGGTTCACCTACGGA15770.23280498264665458No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC15490.22867147629655546No Hit
GCTGCGTTCTTCATCGATGCTGTAAATGTAACACTAGAAGCACCTCGCCGGTTCCCCTACGGA14250.21036594817468787No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG14200.20962782204074162No Hit
GCTGCGTTCTTCATCGATGCATGAGAAACATACAGTGTGTGCCCAGGGAG14190.20948019681395236No Hit
GCTGCGTTCTTCATCGATGCTGCCAGTTGGCTAGGTGGGACTTCCTGGCG13460.19870355525833677No Hit
GCTGCGTTCTTCAGATAGAAGCTCCTCACCAGGAAGTCCTCACACCAGAT13060.19279854618676656No Hit
GCTGCGTTCTTCATCGATGCCTGGTGGAAGTGGGCTTACTCTGCATCCTG12530.18497440916693608No Hit
GCTGCGTTCTTCATCGAAAGTACTGACTTAGCAAGTGCTTCCTGTGCCAC12220.18039802713646919No Hit
GCTGCGTTCTTCATCGATGCCACTGTGCTTTCTCCCAGCCAGGCCCTACT11420.16858800899332882No Hit
GCTGCGTTCTTCATCACAGACAATTGCTGAGAACTGACCCTGGGCTCACA10800.15943524493239503No Hit
GCTGCGTTCTTCATCGATGCCCTGCTGGCTGACATCAGGAAGGGCTTCCA10410.1536778610876141No Hit
GCTGCGTTCTTCATCGATGTGGGAACCAAGACATCCGTTGCTGAAGGTGT10170.150134855644672No Hit
GCTGCGTTCTTCATCGATGCTCCCTACCAAAGAGCAGGTTCACCTACGGA9440.1393582140890564No Hit
GCTGCGTTCTTCATCGATGCAAATGGGCTGGATGTACGCATTGTAGGTGA9180.1355199581925358No Hit
GCTGCGTTCTTCATCGATGTGGCAGCTGTGGAGCCCACAGATGCAAGTGC8550.12621956890481273No Hit
GCTGCGTTCTTCATCATGCTTATTAAGAGCTTGGGCTGGTTTATCCTTTC8120.11987168415287477No Hit
GCTGCGTTCTTCATCGATGCTGTAAATGTAACACTAGAAGCACCTCGCCGGTTCACCTACGGA7810.11529530212240789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)