FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001565403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873371
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT13409315.353498112485989No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG13193115.105951537204692No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10854212.427937268354457No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG302733.4662245483305494No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT256342.9350642510456613No Hit
GCTGCGTTCTTCATCGATTTGTGCTGCTATAACAATATACGACAGAGTGA228492.6161848744691545No Hit
GCTGCGTTCTTCATCGATGCAAATGGAAAGGGTAGTGAAACTTCGATATG196932.254826413975275No Hit
GCTGCGTTCTTCATCGATGTTAGAGCCAAGAGATCCGTTGTTGATAGTTA180812.070254221859897No Hit
GCTGCGTTCTTCATCGCCCCCATAACCTCCTGCCAGTCTGCCTGTGCCAT169641.9423589745938437No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG126961.4536777612263287No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC122431.40180976927331No Hit
GCTGCGTTCTTCATCGATGCCTCCAATCACAGCCAGGACTGCCATGAGGC116941.3389498849858765No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATATGCCAAACCAA105701.210253145570439No Hit
GCTGCGTTCTTCATCACAGACAATTGCTGAGAACTGACCCTGGGCTCACA99251.1364013689485912No Hit
GCTGCGTTCTTCATCGATGCAAACATTCTCAATAAAATACTGGCAAACCG94501.0820144016689357No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG53260.6098210268030425No Hit
GCTGCGTTCTTCATCGATGCAAAAGTCCTCAACAAAATACTGGCAAACCA47910.5485641268143778No Hit
GCTGCGTTCTTATCAGACGATGGCAAATTAATGATTGATGATCAACAATA46790.5357402524242275No Hit
GCTGCGTTCTTCATCGATGCCGAGCCGCAGGTTCACCTACGGA45410.5199394071935065No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA44780.5127259778490469No Hit
GCTGCGTTCTTCATCGATGCCTGGTGGAAGTGGGCTTACTCTGCATCCTG41310.47299486701527754No Hit
GCTGCGTTCTTCATCGATGCGAAAATCCTCAATAAAATATGCCAAACCCA40210.46039999038209417No Hit
GCTGCGTTCTTCATCGATGCTCCCTGTGAGTAGGTTCAGGTTGGGCAGGTTCACCTACGGA39650.45398805318701907No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG39060.4472326193564934No Hit
GCTGCGTTCTTCATCGCCCCCATAACCTCCTGCCAGTCTGCCTGTGCCCT35860.4105929782417781No Hit
GCTGCGTTCTTCATCGATGCAAAGGGAAAGGGTAGTGAAACTTCGATATG35480.4062420208594057No Hit
GCTGCGTTCTTCATCGATGCGCTTGCAATGGAAGCGGGCCACCTTGAGCA33960.3888381913299159No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG29610.33903117918959985No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACAG27310.31269643713839823No Hit
GCTGCGTTCTTCATCGATGCCTCCAATCACAGCCATGACTGCCATGAGGC27030.30949046854086065No Hit
GCTGCGTTCTTCATTCTGTGCAATATCAAAACGGAGAAATATCCATCACA26640.3050250122800047No Hit
GCTGCGTTCTTCATCGATGCCGAGCCTCAGGTTCACCTACGGA25060.286934189479614No Hit
GCTGCGTTCTTCATCGCCCCCATAACCTCCTGCCATTCTGCCTGTGCCCT21440.2454855954685924No Hit
GCTGCGTTCTTCATCACCGACAATTGCTGAGAACTGACCCTGGGCTCACA19560.22395980631369716No Hit
GCTGCGTTCTTCATCGATGCATTAATCCATGGCTAAAACATGACTCATCG19440.22258581977189534No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGTGGCAAGGATCTACG19270.22063933883767609No Hit
GCTGCGTTCTTCATCGATGCCTGGTGTAAGTGGGCTTACTCTGCATCCTG18100.2072429700551083No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG17260.19762506426249554No Hit
GCTGAGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT16470.18857965286230022No Hit
GCTGCGTTCTTCATCGATGCCATTGTGGCTAAGGTACAGCTCCCGCAGGTTCACCTACGGA16300.18663317192808096No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTACG16180.18525918538627914No Hit
GCTGCGTTCTTCATCAAAAGTACTGACTTAGCAAGTGCTTCCTGTGCCAC16110.18445769323689473No Hit
GCTGAGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG15420.17655727062153426No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAAACA15180.17380929753793062No Hit
GCTGCGTTCTTCATCGATGCTGTAAATGTAACACTAGAAGCACCTCGCCGGTTCCCCTACGGA14990.1716338188467444No Hit
GCTGCGTTCTTCATCGATGCCACTGTGCTTTCTCCCAGCCAGGCCCTACT13300.1522835083830354No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC12880.147474555486729No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG11720.13419268558264472No Hit
GATGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG11080.12686475735970165No Hit
GATGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT10950.12537627193941633No Hit
GCTGCGTTCTTCATGATGCAAATGGAAAGGGTAGTGAAACTTCGATATGA9330.10682745362509175No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT8910.10201850072878535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)