FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699669
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACC12098017.29103333147531No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG461106.5902591082354665No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGGCTAA439636.283399721868483No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCGAGGCTCTACGCTTGATG344214.919611987954304No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA247463.5368152655041167No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC229413.2788361353725834No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC222713.1830765690633713No Hit
TCCGTAGGTGAACCTGCGGACCGACAACACGATCTCGGAGCAGATGTGGG194612.78145808946802No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAAGTTTCGGGCCTGCC136131.9456342927870178No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG128641.838583673136869No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN124301.776554342124633No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG116111.6594989916660592No Hit
TCCGTAGGTGAACCTGCGGAGGACAATTCGGCAGTGGCAGTGGTAGTTGT115201.6464928416151066No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGGGCT112361.6059022194780674No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCA99981.4289614088947773No Hit
TCCGTAGGTGAACCTGCGGACAGCTCCACGTTGTTCCTGGGGAGGGGAAA91421.3066178435803215No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAAGTTTCGGGCCTGCC90031.286751306689306No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAATTTTAGGACGTTCTT87661.252878146666495No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTCGCCTTCGGGCTAA86301.2334403839529835No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAATGAGAACGTGCCCTCACGG85731.2252936745804088No Hit
TCCGTAGGTGAACCTGCGAGGACAATGGCCCATGACATTTCAGTGGCTGC81141.1596912254223068No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGAGCGT79531.136680344562929No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGTTTGCTTAATTGCATTA71241.0181957468460088No Hit
TCCGTAGGTGAACCTGTAGGGATTGGGCAGCTCCGTGAGTGTCTCTGTCG53240.7609312403436482No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTTAATTGCACC52090.744494896872664No Hit
TCCGTAGGTGAACCTGCGGAGTAGTGGCATCGATGAAGAACGCAGC50280.7186255214965934No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA49090.7016174791222707No Hit
TCCGTAGGTGAACCTGCGGACGGCATCGATGAAGAACGCAGC48780.6971868126213967No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG46940.670888663067822No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATCGT45930.656453265758523No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAGAGTTCATGCCCGAAAGG43090.6158626436214839No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTGCCCTCTGGGCTTA42660.6097168804105941No Hit
TCCGTAGGTGAACCTGCGGACCCCAAGTACCATGAAGATGGGGATGGGAT42260.6039998913772083No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAATGCTTAGGGGAAATC38640.5522611406250669No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATGAAAAGTGCTTAAC38280.5471158504950199No Hit
TCCGTAGGTGAACCTGCGTGCCTGTGGCTCACTCCAGGGGTGTGTTGCGT37450.5352530982507443No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTACTTGCACC34960.4996648415179178No Hit
TCCGTAGGTGAACCTGCGGAGGAGAACAGCATCGATGAAGAACGCAGC34070.48694454091863437No Hit
TCCGTAGGTGAACCTGCGAATACCTCGATGTTGGGCCTCCCAGTTTCCAG33590.4800841540785714No Hit
TCCGTAGGTGAACCTGCGGAGGGACTTCAATCGCATCGATGAAGAACGCAGC32550.46521998259176844No Hit
TCCGTAGGTGAACCTGCGGACCGAAAGAGCATCGATGAAGAACGCAGC32430.46350488588175265No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC32060.4582166710258708No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGATCAT30960.44249495118405985No Hit
TCCGTAGGTGAACCTGCGGAGGTGAACCTGCATCGATGAAGAACGCAGC30870.4412086286515481No Hit
TCCGTAGGTGAACCTGCGGAGCACGACGGCATCGATGAAGAACGCAGC29230.41776897361466636No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA28510.4074783933545719No Hit
TCCGTAGGTGAACCTGCGGATCGAAAACGTGGGTCGGAGCGGGCATCGATGAAGAACGCAGC24370.3483075568590291No Hit
TCCGTAGGTGAACCTGCGGAGGGGTCATTACAAGTGACCCCGGTTTACCA22040.31500609573955685No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGTCCCCGGTTTACCA21690.3100037303353443No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGAGATAAAGCTGACTTGATCGT21540.3078598594478246No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC19510.27884614010339176No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG18730.2676980114882895No Hit
TCCGTAGGTGAACCTGCGGACACGCTGTGGCTGCATCGATGAAGAACGCAGC17980.2569786570506911No Hit
TCCGTAGGTGAACCTGCGGGGCATGGCCTTCTTCGGCCTGACCGAGTTCC17760.25383431308232896No Hit
TCCGTAGGTGAACCTGCGGGGACAAGGGCATGGGGTGGTAGGAGAAAAGG16580.23696919543384087No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG15140.21638803491365205No Hit
TCCGTAGGTGAACCTGCGGCTCTCTGGCATCGATGAAGAACGCAGC13240.18923233700506953No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC12120.17322476771158934No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGACGAAGCCCACTTGATCAT11460.1637917358065028No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCG10860.1552162522564241No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCATCGATGAAGAACGCAGC10510.15021388685221154No Hit
TCCGTAGGTGAACCTGCGGGGCAGAGGCGGCGGCCGCAGCGGCGCAGCTG10430.14907048904553438No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCAACTTGATCAT10180.14549737089966827No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCCCACTTGGTCAT9380.1340633928328967No Hit
TCCGTAGGTGAACCTGCCCCTGCCAGGGTTTCAGCAGGGCCAAGCTGGAA9380.1340633928328967No Hit
TCCGTAGGTGAACCTGCCACGGCGACAAGCCCTGCCGCGAACAGGAATCC9360.1337775433812274No Hit
TCCGTAGGTGAACCTGCGGACTGCATCGATGAAGAACGCAGC7790.11133836142518819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)