FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001565405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001565405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699669
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT13211818.882928927821585No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA561188.020649764388589No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA446766.385305051388585No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG340124.861155775087934No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG323414.622328558218243No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT295774.227284616011286No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC213413.050156574037152No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA180422.578647903508659No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG172922.4714543591326756No Hit
GCTGCGTTCTTCATCGATGCCGCTTTCAATAGTCGCGTAGGCCTGGCCGT170692.43958214527155No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT127671.8247199747309084No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN123761.7688364069295623No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG110461.5787465215694851No Hit
GCTGCGTTCTTCATCGATGCCCGAAACCAGAACAACTACCACTGCCACTG98151.4028061840670376No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT87461.2500196521498024No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTT87081.2445885125680858No Hit
GCTGCGTTCTTCATCGATGCCGCCAGGTGGTCCCGTCACCTCTTTCCCCT82441.1782714397808105No Hit
GCTGCGTTCTTCATCGATGCGAGTGCCAAGAGATCCGTTGTTGAAAGTTA75371.077223658615717No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG71311.0191962199268512No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG62770.8971385040640646No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGGTCCGTTGTTGAAAGTTT60410.8634082687670885No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATTCGTTGTTGAAAGTTT50650.7239137363524752No Hit
GCTGCGTTCTTCATCGATGCCGTCCGCAGGTTCACCTACGGA48320.6906122752330031No Hit
GCTGCGTTCTTCATCGATGCCTCCAATCACAGCCAGGACTGCCATGAGGC46870.6698881899869795No Hit
GCTGCGTTCTTCATCGATGCCACTACTCCGCAGGTTCACCTACGGA45460.6497358036442946No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT44680.6385876750291923No Hit
GCTGCGTTCTTCATCGATGCTCCGCATGTTCACCTACGGA39180.5599790758201378No Hit
GCTGCGTTCTTCATCGATGCTGCTCAGCATCCCATCCCCATCTTCATGGT34090.48723039037030363No Hit
GCTGCGTTCTTCATCGATTTGTGCTGCTATAACAATATACGACAGAGTGA32160.45964591828421725No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC29400.42019869395385534No Hit
GCTGCGTTCTTCATCGATGCGATTGAAGTCCCTCCGCAGGTTCACCTACGGA29160.41676850053382386No Hit
GCTGCGTTCTTCATCGATGCTGTTCTCCTCCGCAGGTTCACCTACGGA29080.4156251027271467No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG26950.3851821361243674No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA26180.37417693223509974No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTCCGCAGGTTCACCTACGGA25600.36588729813669035No Hit
GCTGCGTTCTTCATCGATGCTCTTTCGGTCCGCAGGTTCACCTACGGA25240.36074200800664313No Hit
GCTGCGTTCTTCATCGATGCCCGCTCCGACCCACGTTTTCGATCCGCAGGTTCACCTACGGA23390.33430093372723385No Hit
GCTGCGTTCTTCATCGATGCCGTCGTGCTCCGCAGGTTCACCTACGGA22720.3247249770963127No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATATGGGCAAATTG20410.2917093654285098No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA17450.24940364658145495No Hit
GCTGCGTTCTTCATCGATGCGCCGGATGGTGTCCTCATCCACCTCCACGC17320.24754562514560458No Hit
GCTGCGTTCTTCATCGATGCAGCCACAGCGTGTCCGCAGGTTCACCTACGGA16110.23025173331961257No Hit
GCTGCGTTCTTCATCCCCCCATGGAAGAAGTGATCCAAGAGGTATTTTCA13880.1983795194584868No Hit
GCTGCGTTCTTCATCGATGCCCGAAACCAGGACAACTACCACTGCCACTG13600.19437762713511675No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC11990.17136674627573895No Hit
GCTGCGTTCTTCATCGATGCCAGAGAGCCGCAGGTTCACCTACGGA11010.15736012314394376No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTG10780.15407285444974694No Hit
GCTGCGTTCTTCATCGATGCTCCACAATGGGCAGGTTCACCTACGGA10200.14578322035133756No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG10100.1443539730929911No Hit
GCTGCGTTCTTCATCGATGCCGCCAGTTGGTCCCGTCACCTCTTTCCCCT9840.14063793022129034No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATGCTGACAAACCA9580.1369218873495896No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA9400.134349242284566No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAGTACTGGCAAACCG9070.12963272633202272No Hit
GCTGAGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT8780.12548790928281803No Hit
GCTGCGTTCTTCATCCTGGTGCAGAACGATATCAAGAGGAAGCTGGCGTA8320.11891337189442436No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGTGGCAAGGATCTACG7970.1139110064902118No Hit
GCTGCGTTCTTCATCGATGCAGTCCGCAGGTTCACCTACGGA7820.11176713560269212No Hit
GCTGCGTTCTTCATCGATGCCTGGTGGAAGTGGGCTTACTCTGCATCCTG7780.11119543669935354No Hit
GCTGCGTTCTTCAAATGCGGGGGCAGGCGTGAAGCTGGCCCGACGCACCC7490.10705061965014886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)