FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001579291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001579291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39291496
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC878579122.360540815244093TruSeq Adapter, Index 1 (100% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCT14061423.578743858467491TruSeq Adapter, Index 1 (100% over 51bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG6937801.7657255911050065TruSeq Adapter, Index 1 (100% over 49bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT4920811.2523855034687403Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT3139640.7990634920085506No Hit
GAGAGAACGCGGTCTGAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA2387610.6076658419928832Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGTCTGAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1809060.4604202395347838TruSeq Adapter, Index 1 (100% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA1657060.4217350237822454No Hit
GCGGGACATGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1595040.40595043772321626TruSeq Adapter, Index 1 (100% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1483320.3775168041451005TruSeq Adapter, Index 1 (100% over 51bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1401300.3566420581186321No Hit
CGGTCTGAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1170900.29800341529373175TruSeq Adapter, Index 1 (100% over 37bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1149930.2926663825678717Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1113360.283359025067409Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT1050120.267263939250366TruSeq Adapter, Index 1 (100% over 41bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1044750.2658972312991086TruSeq Adapter, Index 1 (100% over 49bp)
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG990750.25215379938702254No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC978620.24906661736677063No Hit
TGAGGTAGTAGGTTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGT965740.24578855434773975Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAGTCGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG932470.23732107324190457TruSeq Adapter, Index 1 (100% over 39bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG918410.23374269078479476No Hit
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAG869980.22141686842364056Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC837450.2131377232366006Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA830260.21130781072830618Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGGTCTGAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT825260.21003527073644637TruSeq Adapter, Index 1 (100% over 35bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG798010.20309992778081037No Hit
TTTGGAAATAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC783400.199381565924596TruSeq Adapter, Index 1 (100% over 38bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA749790.19082755209931432TruSeq Adapter, Index 1 (100% over 37bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT749400.19072829397994925TruSeq Adapter, Index 1 (100% over 48bp)
GATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGT731380.18614205984928647TruSeq Adapter, Index 1 (100% over 46bp)
AGAGAACGCGGTCTGAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAG725700.18469645441853372Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA693740.17656237879056577No Hit
TGAAAAGAACTTTGAAGAGAGAGATCGGAAGAGCACACGTCTGAACTCCAG683170.17387222924777412Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA658380.16756297596813316Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA646170.1644554333080115Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGACATGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC646130.1644452529880766TruSeq Adapter, Index 1 (100% over 38bp)
GAACGCGGTCTGAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA633680.16127662840834567Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG621200.15810036858866355No Hit
GCGGGACATGTGGCGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC605440.15408932253432142Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA603710.15364902369713793TruSeq Adapter, Index 1 (100% over 47bp)
GAGAGAACGCGGTCTGAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAG597970.15218814778648285Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGACATGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA591000.15041422703783025TruSeq Adapter, Index 1 (100% over 37bp)
GCGGTCTGAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA576600.14674931186127402TruSeq Adapter, Index 1 (100% over 37bp)
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA562780.14323201132377347No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA541920.13792297447773433Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC539900.13740886832102295TruSeq Adapter, Index 1 (100% over 36bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCA537030.1366784303656954Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA535240.1362228610486096Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG529230.1346932679783941TruSeq Adapter, Index 1 (100% over 49bp)
CGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC528580.13452783777945232TruSeq Adapter, Index 1 (100% over 42bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG519310.13216854863454422Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGAACGCGGTCTGAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT489830.12466565284253875Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT487910.12417699748566459TruSeq Adapter, Index 1 (100% over 35bp)
CGGAGTCGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC485150.12347455541015798TruSeq Adapter, Index 1 (100% over 38bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCCA474730.12082258206712211Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGGG468110.11913773911789971No Hit
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC462120.11761323620765164Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCC460200.11712458085077748Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT445950.113497841873977Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTCTGAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC443020.11275213343874715TruSeq Adapter, Index 1 (100% over 38bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA442410.11259688355974025TruSeq Adapter, Index 1 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC442100.11251798608024495No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC441330.11232201492149853Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAAAGGTATTTTCTGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA437110.11124799116836886Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT418810.10659049479816193No Hit
GCGGGACATGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC410100.10437373013234212TruSeq Adapter, Index 1 (100% over 38bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG402490.10243692426473149Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC401310.10213660482665257No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCAC393780.10022015959891169No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)