FastQCFastQC Report
Mon 18 Sep 2023
EGAF00001579327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001579327
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences43923285
Sequences flagged as poor quality0
Sequence length90
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1710480.3894244248807893TruSeq Adapter, Index 1 (100% over 50bp)
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA1207300.27486559805351535No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG1036880.23606613212103786No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC1011070.23018997782155867No Hit
GGGGAATGCTGGAGATTGTAATGGGTATGGAGACATATCATATAAGTAAT1005810.2289924353335594No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA954940.21741087899049444No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT866770.19733724378766296No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT804920.1832558744183182No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATA748810.17048132898074447No Hit
GGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCT634130.14437217070626662No Hit
GGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTC616650.1403925047955771No Hit
GTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCAT609850.13884435100881004No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT595690.13562054841754206No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG581390.1323648720718407No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT557270.12687347952230804No Hit
GTGGGATTCACCACTTTTCCCATGAAGAGGGGAGACTTGGTATTTTGTTC509700.11604323310517417No Hit
TGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAG493450.11234360089414988No Hit
GGGGGATAGACATGGGTATGGCCTCTAAAAACATGGCCCCAGCAGCTTCA493090.11226163981132102No Hit
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT463930.10562279210218452No Hit
GGGGGATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTT463170.10544976314954585No Hit
GTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA455580.10372175031990434No Hit
GTGGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGG453040.10314346934661194No Hit
GTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCATTGGT452690.10306378496052833No Hit
CTCGAGTGATGTGGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAG445300.10138130606579177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)