FastQCFastQC Report
Wed 22 Mar 2023
EGAF00001579337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001579337
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences59886699
Sequences flagged as poor quality0
Sequence length90
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC5015680.8375282130678132TruSeq Adapter, Index 6 (100% over 50bp)
GGGCTTTATTGGCTTATAGACCTGGGCAGTCCAGGTGTAGATCTGGTTCC1159600.19363231224349164No Hit
CCCCCGAGCATGGAAGTATTTGTCTGAGCCGATGTAATTGGCTTCTCTCA986130.16466594694090586No Hit
GTCGGGTTGGAGGGGCAGGATGGCCCCCAAGTCTCTGTCCTGATCCAAGG850060.14194470795593525No Hit
GTGGGATTCACCACTTTTCCCATGAAGAGGGGAGACTTGGTATTTTGTTC839790.1402298029483976No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG802750.13404479014613913No Hit
CCCGCTTTGTATCCCTGCCCTGAGGGCCTCACAGCCAGATCTCCTGAGAG721900.12054429648894156No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA663590.11080757682102331No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT656460.10961699525298599No Hit
GGGGGATAGACATGGGTATGGCCTCTAAAAACATGGCCCCAGCAGCTTCA632250.1055743613452463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)