FastQCFastQC Report
Wed 15 May 2024
EGAF00001590158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001590158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79246899
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4277440.539761183589026Illumina Single End PCR Primer 1 (100% over 50bp)
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1875860.23671083962540918No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1155140.14576469421219876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)