FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001590236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001590236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3727971
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT830202.2269486538387775TruSeq Adapter, Index 15 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG109340.2932962729592049TruSeq Adapter, Index 15 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAACTCGTATG104530.28039381207632785TruSeq Adapter, Index 15 (97% over 39bp)
TATATATATATATATATATATATATATATATATATATATATATATATATA42000.11266182059892635No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAACCTCGTAT41230.11059635388794602TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)