FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001592111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001592111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33775850
Sequences flagged as poor quality0
Sequence length80
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGTATCATCTCGTAT1662920.4923399411117707TruSeq Adapter, Index 5 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1527750.4523202228811414No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC795780.2356062097623006No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA578680.17132951502330807No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT459570.13606467342790782No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC413290.1223625756272603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)