FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001592668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001592668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678055
Sequences flagged as poor quality0
Sequence length100
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA15650.230807235401258TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATTCATCTCGTA12010.17712427457949576TruSeq Adapter, Index 25 (97% over 39bp)
TGAGATTTAGTGACTTCTTTAGGGAAGAATAATACACACATGGGGACCAG7620.11238026413786494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)